SHARPIN

Gene Symbol SHARPIN
Entrez Gene 81858
Alt Symbol SIPL1
Species Human
Gene Type protein-coding
Description SHANK-associated RH domain interactor
Other Description hSIPL1|shank-associated RH domain-interacting protein|shank-interacting protein-like 1|sharpin
Swissprots Q9H0F6 Q8N2E7 A6NEG3 C0L3L2 Q8IXF5 D3DWL3 Q8TB25 Q8IXF6 Q9BUE4
Accessions CAI72172 EAW82150 EAW82151 EAW82152 EAW82153 EAW82154 EAW82155 EAW82156 Q9H0F6 AB052764 BAC53797 AB052765 BAC53798 AK075518 BAC11666 AL136816 CAB66750 BC002688 AAH02688 BC017230 AAH17230 BC025244 AAH25244 BC034028 AAH34028 BE877614 BI911222 CN260986 DQ583527 FJ655995 ACN38058 NM_030974 NP_112236 NR_038270
Function Component of the LUBAC complex which conjugates linear polyubiquitin chains in a head-to-tail manner to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is proposed to be recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with FAM105B/otulin, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. {ECO:0000269|PubMed:21455173, ECO:0000269|PubMed:21455180, ECO:0000269|PubMed:21455181}.
Subcellular Location Cytoplasm, cytosol {ECO:0000269|PubMed:20179993}. Cell junction, synapse {ECO:0000250}. Note=Enriched at synaptic sites in mature neurons where it colocalizes with SHANK1. {ECO:0000250}.
Tissue Specificity Highly expressed in skeletal muscle and placenta and at lower levels in brain, heart, colon without mucosa, thymus, spleen, kidney, liver, small intestine, lung and peripheral blood leukocytes. Up-regulated in various tumor tissues such as kidney, liver, ovary and pancreas tumors. {ECO:0000269|PubMed:20179993}.

Sharpin (D4P5B) Rabbit mAb - 12541 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4P5B
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Sharpin (hSharpin-Myc/DDK; +), using Sharpin (D4P5B) Rabbit mAb.

Sharpin Antibody - 4444 from Cell Signaling Technology

Host Rabbit
Clonality Polyclonal

SHARPIN (G-18) - sc-98127 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
SHARPIN (G-18): sc-98127. Immunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue showing nuclear staining of cells in non-germinal centers.

SHARPIN (Y-14) - sc-98129 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
SHARPIN (Y-14): sc-98129. Western blot analysis of SHARPIN expression in mouse epididymus tissue extract.

Anti-SHARPIN antibody - ab197853 from Abcam

Prices $384.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis with ab197853 of Immunoprecipitationusing U2OS cells extractsLane 1: Input.Lane 2: Immunoprecipitation with control serum.Lane 3: Immunoprecipitation with ab197853 (5μl)

Anti-SHARPIN antibody - ab125188 from Abcam

Prices $378.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHARPIN antibody (ab125188) at 0.1 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-SHARPIN antibody - ab174545 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-SHARPIN antibody (ab174545) at 100 µg + mouse testis lysate at 35 µg

Anti-SHARPIN antibody - ab69507 from Abcam

Prices $378.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-SHARPIN antibody (ab69507) at 1/500 dilutionLane 1 : SHARPIN transfected 293T cell lysateLane 2 : Non-transfected 293T cell lysateLysates/proteins at 25 µg per lane.SecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Anti-SHARPIN Antibody (aa240-269, AP) - LS-C269310 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SHARPIN Antibody (aa240-269, APC) - LS-C269311 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SHARPIN Antibody (aa240-269, Biotin) - LS-C269312 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SHARPIN Antibody (aa240-269, Azide-free, HRP) - LS-C269315 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SHARPIN Antibody (aa240-269, PE) - LS-C269316 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SHARPIN Antibody (aa239-269) - LS-C325090 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SHARPIN Antibody (aa301-350) - LS-C116843 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SHARPIN antibody LS-C116843 Western blot of HeLa lysate.

Anti-SHARPIN Antibody (aa25-75) - LS-C290226 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SHARPIN by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SHARPIN antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SHARPIN was also immunoprecipitated by rabbit anti-SHARPIN antibody BL12714, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

Anti-SHARPIN Antibody (aa240-269) - LS-C158963 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
SHARPIN Antibody western blot of mouse testis tissue lysates (35 ug/lane). The SHARPIN antibody detected the SHARPIN protein (arrow).

Anti-SHARPIN Antibody (aa240-269, FITC) - LS-C269313 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

SHARPIN Antibody - AF8100 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Western blot shows lysates of U2OS human osteosarcoma cell line and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SHARPIN Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8100) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SHARPIN at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SHARPIN Antibody - NBP2-27186 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal

SHARPIN Antibody (881816) - MAB8100 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 881816
Western blot shows lysates of U2OS human osteosarcoma cell line and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human SHARPIN Monoclonal Antibody (Catalog # MAB8100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for SHARPIN at approximately 43 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

SHARPIN Antibody - NBP1-92386 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemistry-Paraffin: SHARPIN Antibody [NBP1-92386] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.

SHARPIN Antibody - NBP2-04116 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunoprecipitation: SHARPIN Antibody [NBP2-04116] - Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug) and Jurkat (J; 50 ug) cells. Antibodies: Affinity purified rabbit anti-SHARPIN antibody NBP2-04116 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. SHARPIN was also immunoprecipitated by rabbit anti-SHARPIN antibody, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

SHARPIN Antibody - NBP1-79754 from Novus Biologicals

Prices $139.00, $329.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal

SHARPIN Antibody - H00081858-B01P from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
Western Blot: SHARPIN Antibody [H00081858-B01P] - Analysis of SHARPIN expression in HepG2.
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