Anti-SHC (phospho S36) antibody [6E10]
| Name | Anti-SHC (phospho S36) antibody [6E10] |
|---|---|
| Supplier | Abcam |
| Catalog | ab54518 |
| Prices | $385.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 6E10 |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic phosphopeptide corresponding to amino acids surrounding the Ser36 phosphorylation site of human SHC |
| Description | Mouse Monoclonal |
| Gene | SHC1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![All lanes : Anti-SHC (phospho S36) antibody [6E10] (ab54518) at 1 µg/mlLane 1 : HeLa cells stimulated with EGF for 0 mins.Lane 2 : HeLa cells stimulated with EGF for 1 min.Lane 3 : HeLa cells stimulated with EGF for 5 mins.Lane 4 : HeLa cells stimulated with EGF for 15 mins.Lane 5 : HeLa cells stimulated with EGF for 30 mins.Lane 6 : HeLa cells stimulated with EGF for 60 mins.Lysates/proteins at 15 µl per lane.developed using the ECL technique](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/29648_SHC-Primary-antibodies-ab54518-1.jpg)
All lanes : Anti-SHC (phospho S36) antibody [6E10] (ab54518) at 1 µg/mlLane 1 : HeLa cells stimulated with EGF for 0 mins.Lane 2 : HeLa cells stimulated with EGF for 1 min.Lane 3 : HeLa cells stimulated with EGF for 5 mins.Lane 4 : HeLa cells stimulated with EGF for 15 mins.Lane 5 : HeLa cells stimulated with EGF for 30 mins.Lane 6 : HeLa cells stimulated with EGF for 60 mins.Lysates/proteins at 15 µl per lane.developed using the ECL technique
![Overlay histogram showing MCF7 cells stained with ab54518 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54518, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/29649_SHC-Primary-antibodies-ab54518-2.jpg)
Overlay histogram showing MCF7 cells stained with ab54518 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54518, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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