SHC1

Gene Symbol SHC1
Entrez Gene 6464
Alt Symbol SHC, SHCA
Species Human
Gene Type protein-coding
Description SHC (Src homology 2 domain containing) transforming protein 1
Other Description SH2 domain protein C1|SHC (Src homology 2 domain-containing) transforming protein 1|SHC-transforming protein 1|SHC-transforming protein 3|SHC-transforming protein A
Swissprots Q96CL1 Q5T184 O15290 Q8N4K5 Q5T180 P29353 Q5T185 B5BU19 D3DV78 Q5T183 Q5T186
Accessions AAM61859 AAM61860 CAA70977 EAW53167 EAW53168 EAW53169 EAW53170 EAW53171 P29353 AB208849 BAD92086 AB451255 BAG70069 AB451379 BAG70193 AK096169 AK130014 AK225332 AK292143 BAF84832 AK296786 BAG59364 AK315842 BAF98733 BC011813 BC014158 AAH14158 BC017283 BC033925 AAH33925 BX647136 BX647149 HQ448284 ADQ32764 U73377 AAB49972 X68148 CAA48251 XM_005245449 XP_005245506 XM_005245451 XP_005245508 XM_011509892 XP_011508194 XM_011509893 XP_011508195 XM_011509894 XP_011508196 XM_011509895 XP_011508197 XM_011509896 XP_011508198 XM_011509897 XP_011508199 XM_011509898 XP_011508200 NM_001130040 NP_001123512 NM_001130041 NP_001123513 NM_001202859 NP_001189788 NM_003029 NP_003020 NM_183001 NP_892113
Function Signaling adapter that couples activated growth factor receptors to signaling pathways. Participates in a signaling cascade initiated by activated KIT and KITLG/SCF. Isoform p46Shc and isoform p52Shc, once phosphorylated, couple activated receptor tyrosine kinases to Ras via the recruitment of the GRB2/SOS complex and are implicated in the cytoplasmic propagation of mitogenic signals. Isoform p46Shc and isoform p52Shc may thus function as initiators of the Ras signaling cascade in various non-neuronal systems. Isoform p66Shc does not mediate Ras activation, but is involved in signal transduction pathways that regulate the cellular response to oxidative stress and life span. Isoform p66Shc acts as a downstream target of the tumor suppressor p53 and is indispensable for the ability of stress-activated p53 to induce elevation of intracellular oxidants, cytochrome c release and apoptosis. The expression of isoform p66Shc has been correlated with life span (By similarity). Participates in s
Subcellular Location Isoform p66Shc: Mitochondrion {ECO:0000250}. Note=In case of oxidative conditions, phosphorylation at 'Ser-36' of isoform p66Shc, leads to mitochondrial accumulation. {ECO:0000250}.
Tissue Specificity Widely expressed. Expressed in neural stem cells but absent in mature neurons.
Top Pathways Natural killer cell mediated cytotoxicity, Estrogen signaling pathway, Chronic myeloid leukemia, MicroRNAs in cancer, Insulin signaling pathway

Phospho-Shc (Tyr239/240) Antibody - 2434 from Cell Signaling Technology

Prices $109.00, $287.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HepG2 cells, untreated or EGF-treated (100 ng/ml) 18 hours of serum-starvation, and Jurkat cells, untreated or treated with anti-CD3 antibody (1 µg/ml for 10 minutes), using Phospho-Shc (Tyr239/240) Antibody (upper) or Shc antibody #2432 (lower).

Phospho-Shc (Tyr317) Antibody - 2431 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from A431 and HepG2 cells, untreated or EGF-treated (100 ng/ml) after 24 hours of serum-starvation, using Phospho-Shc (Tyr317) Antibody (upper) or Shc antibody (lower).

Shc Antibody - 2432 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HepG2 and Jurkat cells, using Shc Antibody.

Shc (B-9) - sc-393717 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Shc (B-9): sc-393717. Western blot analysis of Shc expression in SK-N-SH (A), SK-BR-3 (B) and H4 (C) whole cell lysates and SK-BR-3 nuclear extract (D).

Shc (F-7) - sc-398163 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Shc (F-7): sc-398163. Western blot analysis of Shc expression in MCF7 whole cell lysate.

Shc (H-11) - sc-398289 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Shc (H-11): sc-398289. Western blot analysis of Shc expression in MCF7 (A) and H4 (B) whole cell lysates.

Shc (PG-797) - sc-967 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Shc (PG-797): sc-967. Western blot analysis of Shc expression in MCF7 whole cell lysate.

Shc (C-20) - sc-288 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Shc (C-20): sc-288. Immunofluorescence staining of methanol-fixed MCF7 cells showing cytoplasmic staining.

Shc (H-108) - sc-1695 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Shc (H-108): sc-1695. Immunofluorescence staining of methanol-fixed SK-BR-3 cells showing cytoplasmic staining.

p-Shc (73.Tyr 349/350) - sc-293148 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Western blot analysis of Shc phosphorylation in untreated (A,C) and EGF treated (B,D) HEK293 whole cell lysates. Antibodies tested include p-Shc (73.Tyr 349/350): sc-293148 (A,B) and Shc (H-108): sc-1695 (C,D).

p-Shc (Tyr 239/240) - sc-23764 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Shc (Tyr 317) - sc-18075 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

p-Shc (Tyr 317) - sc-101797 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Shc (Tyr 317): sc-101797. Western blot analysis of phosphorylated Shc expression in untreated (A) and EGF-treated (B) 293 whole cell lysates.

p-Shc (Tyr 239) - sc-101796 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Shc (Tyr 239/240): sc-101796. Western blot analysis of phosphorylated Shc expression in untreated (A) Insulin-treated (B) and EGF-treated (C) 293 whole cell lysates.

p-Shc (6E10) - sc-81520 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal

p-Shc (1E3) - sc-81519 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-Shc (1E3): sc-81519. Western blot analysis of Shc phosphorylation in untreated (A) EGF-stimulated (B) or pervanadate-treated (C) A549 whole cell lysates.

p-Shc (15E11) - sc-81518 from Santa Cruz Biotechnology

Prices $279.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
p-Shc (15E11): sc-81518. Western blot analysis of Shc phosphorylation in non-transfected: sc-117752 (A) and mouse Shc transfected: sc-123541 (B) 293T whole cell lysates.

Anti-SHC (phospho S36) antibody [6E10] - ab54518 from Abcam

Prices $385.00
Sizes 50 µg
Host Mouse
Clonality Monoclonal
Clone 6E10
All lanes : Anti-SHC (phospho S36) antibody [6E10] (ab54518) at 1 µg/mlLane 1 : HeLa cells stimulated with EGF for 0 mins.Lane 2 : HeLa cells stimulated with EGF for 1 min.Lane 3 : HeLa cells stimulated with EGF for 5 mins.Lane 4 : HeLa cells stimulated with EGF for 15 mins.Lane 5 : HeLa cells stimulated with EGF for 30 mins.Lane 6 : HeLa cells stimulated with EGF for 60 mins.Lysates/proteins at 15 µl per lane.developed using the ECL technique

Anti-SHC (phospho Y239 + Y240) antibody [EP419] - ab109455 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP419
All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/5000 dilutionLane 1 : 293T cell lysates, EGF treated with single phospho (pY239) peptideLane 2 : 293T cell lysates, EGF treated with single phospho (pY240) peptideLane 3 : 293T cell lysates, EGF treated with Double phospho (pY239/pY240) peptideLane 4 : 293T cell lysates, EGF treated with non-phospho peptide blockingLane 5 : 293T cell lysates, EGF treatedLysates/proteins at 10 µg per lane.

Anti-SHC (phospho Y317) antibody - ab15040 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHC (phospho Y317) antibody (ab15040) at 1/250 dilutionLane 1 : RIPA lysates from HFF cellsLane 2 : RIPA lysates from HFF cells stimulated with 50ng/ml EGF for 10 minutes

Anti-SHC (phospho Y349) antibody - ab131449 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol fixed HeLa cells labelling SHC (phospho Y349) with ab131449 antibody at a dilution of 1/100.

Anti-SHC (phospho Y349) antibody - ab194802 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHC (phospho Y349) antibody (ab194802) at 1/500 dilutionLane 1 : Untreated HepG2 cell extractLane 2 : EGF-treated HepG2 cell extract

Anti-SHC (phospho Y349) antibody - ab51168 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHC (phospho Y349) antibody (ab51168) at 1/500 dilutionLane 1 : 293 cell extract treated with EGF (200ng/ml, 30min)Lane 2 : 293 cell extract treated with EGF (200ng/ml, 30min) with immunising peptide

Anti-SHC (phospho Y427) antibody - ab194782 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHC (phospho Y427) antibody (ab194782) at 1/500 dilutionLane 1 : Untreated 293 cell extractLane 2 : PMA treated 293 cell extract

Anti-SHC (phospho Y427) antibody - ab51170 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHC (phospho Y427) antibody (ab51170) at 1/500 dilutionLane 1 : Hela cell extract treated with Calyculin A (50ng/ml, 15min) with immunising peptideLane 2 : Hela cell extract treated with Calyculin A (50ng/ml, 15min)
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