All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/5000 dilutionLane 1 : 293T cell lysates, EGF treated with single phospho (pY239) peptideLane 2 : 293T cell lysates, EGF treated with single phospho (pY240) peptideLane 3 : 293T cell lysates, EGF treated with Double phospho (pY239/pY240) peptideLane 4 : 293T cell lysates, EGF treated with non-phospho peptide blockingLane 5 : 293T cell lysates, EGF treatedLysates/proteins at 10 µg per lane.
All lanes : Anti-SHC (phospho Y239 + Y240) antibody [EP419] (ab109455) at 1/500 dilutionLane 1 : 293T cell lysates, untreatedLane 2 : 293T cell lysates, treated with EGFLysates/proteins at 10 µg per lane.