Anti-SHP1 antibody
| Name | Anti-SHP1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2020 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IP WB ICC/IF ICC/IF |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 300 - 400 of Human SHP1 |
| Description | Rabbit Polyclonal |
| Gene | PTPN6 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab2020 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).Picture of a B cell follicle.The antibody shows strong staining of mantle zone cells and some staining of a few cells in the light zone of the germinal centre. This is in keeping with published data (see Khoury J et al and Oka T et al.).
ICC/IF image of ab2020 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab2020, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
All lanes : Anti-SHP1 antibody (ab2020) at 1 µg/mlLane 1 : PTPN6 - Recombinant Protein (Human) at 0.1 µgLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µgLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.
![SHP1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to SHP1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2020.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 68kDa; SHP1.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_4/29818_SHP1-Primary-antibodies-ab2020-11.jpg)
SHP1 was immunoprecipitated using 0.5mg Jurkat whole cell extract, 5µg of Rabbit polyclonal to SHP1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2020.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 68kDa; SHP1.
All lanes : Anti-SHP1 antibody (ab2020) at 1/2000 dilutionLane 1 : PTP1B (PTPN1)Lane 2 : TCPTP (PTPN2)Lane 3 : PTPH1 (PTPN3)Lane 4 : MEG1 (PTPN4)Lane 5 : STEP (PTPN5)Lane 6 : SHP1 (PTPN6)Lane 7 : HePTP (PTPN7)Lane 8 : SHP2 (PTPN11)Lane 9 : BDP1 (PTPN18)
ab2020 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).The antibody shows staining of T cells in the interfollicullar area. This is in keeping with published data (see Khoury J et al and Oka T et al.).
Product References
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Differential cell fates induced by all-trans retinoic acid-treated HL-60 human - Differential cell fates induced by all-trans retinoic acid-treated HL-60 human
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Angiotensin II and endothelin-1 augment the vascular complications of diabetes - Angiotensin II and endothelin-1 augment the vascular complications of diabetes
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