PTPN6

Gene Symbol PTPN6
Entrez Gene 5777
Alt Symbol HCP, HCPH, HPTP1C, PTP-1C, SH-PTP1, SHP-1, SHP-1L, SHP1
Species Human
Gene Type protein-coding
Description protein tyrosine phosphatase, non-receptor type 6
Other Description hematopoietic cell phosphatase|hematopoietic cell protein-tyrosine phosphatase|protein-tyrosine phosphatase 1C|protein-tyrosine phosphatase SHP-1|tyrosine-protein phosphatase non-receptor type 6
Swissprots Q9UK67 P29350 G3V0F8 Q969V8 A8K306
Accessions AAA82879 AAA82880 AAB51322 AAB51323 BAC81774 BAC81775 CBX47486 CBX47487 EAW88700 EAW88701 EAW88702 EAW88703 EAW88704 P29350 AF178946 AAD53317 AK223629 BAD97349 AK290421 BAF83110 AK298470 BAG60682 AK300167 BAG61949 AK308230 AK309037 AK313379 BAG36177 BC002523 AAH02523 BC007667 AAH07667 BG754792 BM006699 BM742181 BT007390 AAP36054 CR986427 DA431502 DA868001 DC297194 DQ891946 ABM82872 DQ895133 ABM86059 M74903 AAA35963 M77273 AAA36610 XM_006718994 XP_006719057 XM_011520988 XP_011519290 XM_011520989 XP_011519291 XR_931520 NM_002831 NP_002822 NM_080548 NP_536858 NM_080549 NP_536859
Function Modulates signaling by tyrosine phosphorylated cell surface receptors such as KIT and the EGF receptor/EGFR. The SH2 regions may interact with other cellular components to modulate its own phosphatase activity against interacting substrates. Together with MTUS1, induces UBE2V2 expression upon angiotensin II stimulation. Plays a key role in hematopoiesis. {ECO:0000269|PubMed:11266449}.
Subcellular Location Cytoplasm. Nucleus. Note=In neurons, translocates into the nucleus after treatment with angiotensin II (By similarity). Shuttles between the cytoplasm and nucleus via its association with PDPK1. {ECO:0000250}.
Tissue Specificity Isoform 1 is expressed in hematopoietic cells. Isoform 2 is expressed in non-hematopoietic cells.
Top Pathways Adherens junction, Jak-STAT signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, B cell receptor signaling pathway

Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb - 8849 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D11G5
Western blot analysis of extracts from various cell lines, untreated (-) or treated (+) with λ phosphatase, using Phospho-SHP-1 (Tyr564) (D11G5) Rabbit mAb (upper) and SHP-1 (C14H6) Rabbit mAb #3759 (lower). The JCaM1.6 cell line is a Lck kinase-deficient derivative of the Jurkat cell line. Note the corresponding reduction in phospho-SHP-1 (Tyr564) protein in JCaM1.6 cells, relative to wild-type (Jurkat) cells.

SHP-1 (C14H6) Rabbit mAb - 3759 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C14H6
Western blot analysis of extracts from Jurkat, 32D and BaF3 cells using SHP-1 (C14H6) Rabbit mAb.

SH-PTP1 (D-7) - sc-271329 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SH-PTP1 (D-7): sc-271329. Western blot analysis of SH-PTP1 expression in U-937 whole cell lysate.

SH-PTP1 (PTY6) - sc-52885 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
SH-PTP1 (PTY6): sc-52885. Western blot analysis of SH-PTP1 expression in HEL 92.1.7 (A) and TF-1 (B) whole cell lysates.

SH-PTP1 (D-11) - sc-7289 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SH-PTP1 (D-11): sc-7289. Immunoperoxidase staining of formalin fixed, paraffin-embedded human tonsil tissue showing cytoplasmic staining of follicle and non-follicle cells. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

SH-PTP1 (C-19) - sc-287 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SH-PTP1 (C-19)-G: sc-287-G. Western blot analysis of SH-PTP1 expression in U-937 (A), HL-60 (B) and Jurkat (C) whole cell lysates.

SH-PTP1 (G-20) - sc-30810 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SH-PTP1 (G-20): sc-30810. Western blot analysis of SH-PTP1 expression in HL-60 whole cell lysate.

SH-PTP1 (H-65) - sc-33162 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SH-PTP1 (H-65): sc-33162. Western blot analysis of SH-PTP1 expression in Jurkat whole cell lysate.

SH-PTP1 (PTY11) - sc-56964 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
SH-PTP1 (PTY11): sc-56964. Western blot analysis of SH-PTP1 expression in HL-60 (A), HEL 92.1.7 (B) and TF-1 (C) whole cell lysates.

SH-PTP1 (PTY15) - sc-52890 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
SH-PTP1 (PTY15): sc-52890. Western blot analysis of SH-PTP1 expression in HL-60 (A) and HEL 92.1.7 (B) whole cell lysates.

p-SH-PTP1 (82.Tyr 536) - sc-135782 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-SH-PTP1 (14.Tyr 536) - sc-135780 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

p-SH-PTP1 (5.Tyr 536) - sc-135781 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-SHP1 (phospho S591) antibody - ab41436 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Lanes 1 - 2 : anti-SHP1 (C-terminal) Lanes 3 - 6 : Anti-SHP1 (phospho S591) antibody (ab41436) at 1/1000 dilutionLane 1 : human Jurkat cells treated with 1 mM pervanadate for 30 min. Lane 2 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to lambda phosphatase before probing Lane 3 : human Jurkat cells treated with 1 mM pervanadate for 30 min. Lane 4 : human Jurkat cells treated with 1 mM pervanadate for 30 min, blot exposed to lambda phosphatase before probing Lane 5 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with phospho-Ser591-SHP1 peptide Lane 6 : human Jurkat cells treated with 1 mM pervanadate for 30 min. with non specific phospho-serine peptide

Anti-SHP1 (phospho Y536) antibody - ab51171 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab51171 at 1/50 dilution staining SHP1 in human breast carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

Anti-SHP1 (phospho Y536) antibody - ab41437 from Abcam

Prices $379.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab41437 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab41437 at 1/200 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SHP1 (phospho Y536) antibody - ab131500 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Immunofluorescent analysis of methanol-fixed HeLa cells labelling SHP1 (phospho Y536) with ab131500 at 1/100 dilution.

Anti-SHP1 (phospho Y564) antibody - ab79294 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHP1 (phospho Y564) antibody (ab79294) at 1/1000 dilutionLane 1 : extracts from COS7 cells, treated with EGF (200 ng/ml, 30 mins)Lane 2 : extracts from COS7 cells, treated with EGF (200 ng/ml, 30 mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-SHP1 (phospho Y564) antibody - ab192669 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHP1 (phospho Y564) antibody (ab192669) at 1/500 dilutionLane 1 : Extracts from COS7 cells treated with EGFLane 2 : Extracts from COS7 cells treated with EGF with antigen-specific peptide

Anti-SHP1 antibody - ab2020 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
ab2020 was used in immunohistochemistry with paraffin embedded sections of human tonsil, using DAB as a chromogen (brown). Counterstaining of nuclei was performed with haemotoxylin (blue).Picture of a B cell follicle.The antibody shows strong staining of mantle zone cells and some staining of a few cells in the light zone of the germinal centre. This is in keeping with published data (see Khoury J et al and Oka T et al.).

Anti-SHP1 antibody - ab131537 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SHP1 antibody (ab131537) at 1/500 dilution + 293 cell extract

Anti-SHP1 antibody - ab55356 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SHP1 antibody (ab55356) at 1/500 dilutionLane 1 : extracts from NIH/3T3 cells with no immunizing peptideLane 2 : extracts from NIH/3T3 cells with immunizing peptide

Anti-SHP1 antibody - ab60268 from Abcam

Prices $378.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-SHP1 antibody (ab60268) at 0.1 µg/ml + Human Liver lysate (35µg protein in RIPA buffer)

Anti-SHP1 antibody - ab61397 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-SHP1 antibody (ab61397) at 0.2 µg/ml + Jurkat cell lysate (35µg protein in RIPA buffer).

Anti-SHP1 antibody [14D5] - ab189299 from Abcam

Prices $376.00
Sizes 50 µl
Host Mouse
Clonality Monoclonal
Clone 14D5
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue, labeling SHP1 with ab189299 at 1/200 dilution.
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