Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human ovarian carcinoma tissue labeling SIAH Interacting Protein with ab176730 at 1/200 dilution (1µg/ml), with DAB detection.
Detection of SIAH Interacting Protein in Immunoprecipitaties of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab176730 at 3 µg/mg lysate for IP and at 0.4 µg/ml for subsequent Western blot detection.Detection: Chemiluminescence with exposure time of 3 seconds.
All lanes : Anti-SIAH Interacting Protein antibody (ab176730) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgLane 5 : NIH3T3 whole cell lysate at 50 µgdeveloped using the ECL technique