![All lanes : Anti-SIKE1 antibody [EPR14692] (ab183509) at 1/20000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : Hela cell lysateLane 3 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/84_ab183509-215174-ab183509.jpg)
All lanes : Anti-SIKE1 antibody [EPR14692] (ab183509) at 1/20000 dilutionLane 1 : SH-SY5Y cell lysateLane 2 : Hela cell lysateLane 3 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin embedded Human kidney tissue labeling SIKE1 with ab183509 at 1/50.
Lysate from 293 cells was immunoprecipitated with ab183509 at a 1/50 dilution. For the subsequent blot, ab183509 used at a 1/10000 dilution with an anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at a 1/1500 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST. Lane 2: TBS instead of lysate.