All lanes : Anti-SIKE1 antibody [EPR14692-1] (ab186418) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Anti-SIKE1 antibody [EPR14692-1] (ab186418) at 1/1000 dilution + PC3 cell lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab186418 at 1/50 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.