Anti-SIRP alpha antibody
| Name | Anti-SIRP alpha antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53721 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ELISA IHC-P ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide derived from human SIRP-alpha1 |
| Description | Rabbit Polyclonal |
| Gene | SIRPA |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SIRP alpha antibody (ab53721) at 1/500 dilutionLane 1 : Extracts from HepG2 cellsLane 2 : Extracts from HepG2 cells with immunizing peptide
Ab53721 at 1/50 dilution, staining paraffin embedded human brain, in the presence (right) and absence (left) of blocking peptide.
ICC/IF image of ab53721 stained Mcf7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53721, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
SIRPalpha/CD172a and FHOD1 are unique markers of littoral cells, a recently - SIRPalpha/CD172a and FHOD1 are unique markers of littoral cells, a recently
Ogembo JG, Milner DA Jr, Mansfield KG, Rodig SJ, Murphy GF, Kutok JL, Pinkus GS, Fingeroth JD. J Immunol. 2012 May 1;188(9):4496-505.
Escherichia coli K1 promotes the ligation of CD47 with thrombospondin-1 to - Escherichia coli K1 promotes the ligation of CD47 with thrombospondin-1 to
Mittal R, Gonzalez-Gomez I, Prasadarao NV. J Immunol. 2010 Sep 1;185(5):2998-3006.