All lanes : Anti-SIRP alpha antibody [EPR16264] (ab191419) at 1/5000 dilutionLane 1 : THP1 cell lysateLane 2 : SW480 cell lysateLane 3 : Human fetal brain lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Flow Cytometric analysis of THP1 cells (2% paraformaldehyde-fixed) labeling SIRP alpha with ab191419 at 1/50 dilution (red) or a Rabbit monoclonal IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution
All lanes : Anti-SIRP alpha antibody [EPR16264] (ab191419) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : PC12 cell lysateLane 3 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution