Anti-SIRT1 antibody
| Name | Anti-SIRT1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab12193 |
| Prices | $395.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Dog, Human |
| Antigen | Synthetic peptide: ATRQELTDV NYPSDKS (with C-terminal added lysine), corresponding to C terminal amino acids 722-737 of Mouse SIRT1 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | SIRT1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab12193 staining SIRT1 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for 60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation of rSCs with RSV led to an increase of SIRT1 expression in densitometry analysis.
Anti-SIRT1 antibody (ab12193) at 1/500 dilution (for 1 hour at 21°C) + Whole tissue lysate of mouse jejunum and ileum at 10 µgSecondaryAn HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
SIRT1 signalling protects mouse oocytes against oxidative stress and is - SIRT1 signalling protects mouse oocytes against oxidative stress and is
Di Emidio G, Falone S, Vitti M, D'Alessandro AM, Vento M, Di Pietro C, Amicarelli F, Tatone C. Hum Reprod. 2014 Sep;29(9):2006-17.
Loss of Sirt1 function improves intestinal anti-bacterial defense and protects - Loss of Sirt1 function improves intestinal anti-bacterial defense and protects
Lo Sasso G, Ryu D, Mouchiroud L, Fernando SC, Anderson CL, Katsyuba E, Piersigilli A, Hottiger MO, Schoonjans K, Auwerx J. PLoS One. 2014 Jul 11;9(7):e102495.
Promoting myelination in an in vitro mouse model of the peripheral nervous - Promoting myelination in an in vitro mouse model of the peripheral nervous
Stettner M, Wolffram K, Mausberg AK, Albrecht P, Derksen A, Methner A, Dehmel T, Hartung HP, Dietrich H, Kieseier BC. PLoS One. 2013 Jun 7;8(6):e66079.
SIRT1 promotes thyroid carcinogenesis driven by PTEN deficiency. - SIRT1 promotes thyroid carcinogenesis driven by PTEN deficiency.
Herranz D, Maraver A, Canamero M, Gomez-Lopez G, Inglada-Perez L, Robledo M, Castelblanco E, Matias-Guiu X, Serrano M. Oncogene. 2013 Aug 22;32(34):4052-6.
SIRT1 stabilizes PML promoting its sumoylation. - SIRT1 stabilizes PML promoting its sumoylation.
Campagna M, Herranz D, Garcia MA, Marcos-Villar L, Gonzalez-Santamaria J, Gallego P, Gutierrez S, Collado M, Serrano M, Esteban M, Rivas C. Cell Death Differ. 2011 Jan;18(1):72-9.
Resveratrol-mediated SIRT-1 interactions with p300 modulate receptor activator of - Resveratrol-mediated SIRT-1 interactions with p300 modulate receptor activator of
Shakibaei M, Buhrmann C, Mobasheri A. J Biol Chem. 2011 Apr 1;286(13):11492-505.
Resveratrol-mediated SIRT-1 interactions with p300 modulate receptor activator of - Resveratrol-mediated SIRT-1 interactions with p300 modulate receptor activator of
Shakibaei M, Buhrmann C, Mobasheri A. J Biol Chem. 2011 Apr 1;286(13):11492-505.
Endotoxin uptake by S1 proximal tubular segment causes oxidative stress in the - Endotoxin uptake by S1 proximal tubular segment causes oxidative stress in the
Kalakeche R, Hato T, Rhodes G, Dunn KW, El-Achkar TM, Plotkin Z, Sandoval RM, Dagher PC. J Am Soc Nephrol. 2011 Aug;22(8):1505-16.
The first bromodomain of the testis-specific double bromodomain protein Brdt is - The first bromodomain of the testis-specific double bromodomain protein Brdt is
Berkovits BD, Wolgemuth DJ. Dev Biol. 2011 Dec 15;360(2):358-68.
Complete lack of vitamin C intake generates pulmonary emphysema in senescence - Complete lack of vitamin C intake generates pulmonary emphysema in senescence
Koike K, Kondo Y, Sekiya M, Sato Y, Tobino K, Iwakami SI, Goto S, Takahashi K, Maruyama N, Seyama K, Ishigami A. Am J Physiol Lung Cell Mol Physiol. 2010 Jun;298(6):L784-92. doi: