SIRT1

Gene Symbol SIRT1
Entrez Gene 23411
Alt Symbol SIR2L1
Species Human
Gene Type protein-coding
Description sirtuin 1
Other Description NAD-dependent deacetylase sirtuin-1|NAD-dependent protein deacetylase sirtuin-1|SIR2-like protein 1|SIR2alpha|hSIR2|hSIRT1|regulatory protein SIR2 homolog 1|sir2-like 1|sirtuin type 1
Swissprots Q9GZR9 Q5JVQ0 Q9Y6F0 Q2XNF6 Q96EB6
Accessions ABB72675 CAF86815 CAL00153 CAL48196 CAN36290 CAV28860 EAW54258 EAW54259 Q96EB6 AA452304 AF083106 AAD40849 AF235040 AAG38486 AK027686 BAG51365 AK074805 BAG52006 AK289743 BAF82432 AL136741 BC012499 AAH12499 BX648554 DB162635 JQ768366 AFI98394 XM_005269663 XP_005269720 XM_006717737 XP_006717800 XM_011539561 XP_011537863 NM_001142498 NP_001135970 NM_012238 NP_036370
Function NAD-dependent protein deacetylase that links transcriptional regulation directly to intracellular energetics and participates in the coordination of several separated cellular functions such as cell cycle, response to DNA damage, metobolism, apoptosis and autophagy. Can modulate chromatin function through deacetylation of histones and can promote alterations in the methylation of histones and DNA, leading to transcriptional repression. Deacetylates a broad range of transcription factors and coregulators, thereby regulating target gene expression positively and negatively. Serves as a sensor of the cytosolic ratio of NAD(+)/NADH which is altered by glucose deprivation and metabolic changes associated with caloric restriction. Is essential in skeletal muscle cell differentiation and in response to low nutrients mediates the inhibitory effect on skeletal myoblast differentiation which also involves 5'-AMP-activated protein kinase (AMPK) and nicotinamide phosphoribosyltransferase (NAMPT).
Subcellular Location Nucleus, PML body. Cytoplasm. Nucleus {ECO:0000269|PubMed:20955178}. Note=Recruited to the nuclear bodies via its interaction with PML. Colocalized with APEX1 in the nucleus. May be found in nucleolus, nuclear euchromatin, heterochromatin and inner membrane. Shuttles between nucleus and cytoplasm (By similarity). Colocalizes in the nucleus with XBP1 isoform 2 (PubMed:20955178). {ECO:0000250|UniProtKB:Q923E4, ECO:0000269|PubMed:20955178}.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:10381378}.
Top Pathways Amphetamine addiction, MicroRNAs in cancer, AMPK signaling pathway, Glucagon signaling pathway

Phospho-SirT1 (Ser47) Antibody - 2314 from Cell Signaling Technology

Prices $287.00, $678.00
Sizes 100 µl (20 western blots), 300 µl (60 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of 293 cell lysates treated or untreated with λ phosphatase, using Phospho-SirT1 (Ser47) Antibody #2314 (upper) or SirT1 Antibody #2310 (lower).

Phospho-SirT1 (Ser27) Antibody - 2327 from Cell Signaling Technology

Prices $287.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of 293 and HCT15 cell lysates treated or untreated with lambda phosphatase using Phospho-SirT1 (Ser27) Antibody #2327 (upper) or SirT1 Antibody #2310 (lower).

SirT1 (1F3) Mouse mAb - 8469 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Mouse
Clonality Monoclonal
Clone 1F3
Western blot analysis of extracts from SirT1 wild-type (WT) and knockout (KO) mouse embryo fibroblasts (MEF) using SirT1 (1F3) Mouse mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). Wild-type and knockout MEFs were a gift from Wenyi Wei at the Harvard Medical School.

SirT1 (D1D7) Rabbit mAb - 9475 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D1D7
Western blot analysis of extracts from various cell lines using SirT1 (D1D7) Rabbit mAb.

SirT1 Antibody - 2310 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of lysates from 293, HCT15 and RD-ES cells, using SirT1 Antibody.

SirT1 (C14H4) Rabbit mAb - 2496 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C14H4
Western blot analysis of extracts from various cell types using SirT1 (C14H4) Rabbit mAb.

SirT1 (D739) Antibody - 2493 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell lines using SirT1 (D739) Antibody.

SIRT1 (B-10) - sc-74504 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SIRT1 (B-10): sc-74504. Western blot analysis of SIRT1 expression in K-562 nuclear extract (A) and BJAB (B) and MES-SA/Dx5 (C) whole cell lysates.

SIRT1 (C-20) - sc-19858 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SIRT1 (C-20): sc-19858. Western blot analysis of SIRT1 expression in non-transfected: sc-117750 (A) and human SIRT1 transfected: sc-113797 (B) whole cell lysates.

SIRT1 (B-7) - sc-74465 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SIRT1 (B-7): sc-74465. Western blot analysis of SIRT1 expression in K-562 nuclear extract (A) and BJAB (B), MES-SA/Dx5 (C) and F9 (D) whole cell lysates.

SIRT1 (H-300) - sc-15404 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
SIRT1 (H-300): sc-15404. Western blot analysis of SIRT1 expression in F9 whole cell lysate.

SIRT1 (33.36) - sc-135792 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

SIRT1 (P-20) - sc-19857 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
SIRT1 (P-20): sc-19857. Western blot analysis of SIRT1 expression in F9 whole cell lysate (A) and rat testis tissue extract (B).

SIRT1 (11.69) - sc-135791 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal

Anti-SIRT1 (phospho S27) antibody [EP2850Y] - ab76029 from Abcam

Prices $313.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP2850Y
All lanes : Anti-SIRT1 (phospho S27) antibody [EP2850Y] (ab76029) at 1/10000 dilutionLane 1 : 293T cell lysates, untreatedLane 2 : 293T cell lysates, treated with Calyculin ALysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-SIRT1 (phospho S47) antibody [EPR2849Y] - ab76039 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR2849Y
All lanes : Anti-SIRT1 (phospho S47) antibody [EPR2849Y] (ab76039) at 1/2000 dilutionLane 1 : 293 cell lysateLane 2 : 293 cell lysate treated with LPLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SIRT1 (phospho T530) antibody [EPNCIR153] - ab156585 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPNCIR153
All lanes : Anti-SIRT1 (phospho T530) antibody [EPNCIR153] (ab156585) at 1/1000 dilutionLane 1 : 293T cell lysates Lane 2 : 293T cell lysates treated with Calyculin ALysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SIRT1 antibody - ab12193 from Abcam

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab12193 staining SIRT1 in pure rat Schwann cells by ICC/IF (Immunocytochemistry/immunofluorescence) after resveratol (RSV) treatment. Cells were fixed with 4% PFA blocked with 10% Goat serum/ 0.1% Triton x-100/ 0.1% BSA in PBS for 60 minutes at 21°C followed by 10% Goat serum/ 0.5% Triton X-100/ 0.01% BSA in PBS for 15 minutes at 21°C. Samples were incubated with primary antibody (1/100 in PBS + 10% goat serum) overnight at 21°C. An Alexa Fluor®488-conjugated goat anti-mouse IgG polyclonal (1/400) was used as the secondary antibody. Stimulation of rSCs with RSV led to an increase of SIRT1 expression in densitometry analysis.

Anti-SIRT1 antibody - ab7343 from Abcam

Prices $398.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SIRT1 antibody (ab7343) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - Vector Transfected ControlLane 2 : HEK293 Whole Cell Lysate Transiently Overexpressing SIRT1 Lysates/proteins at 5 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-SIRT1 antibody - ab13749 from Abcam

Prices $391.00
Sizes 200 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SIRT1 antibody (ab13749) at 5 µg/mlLane 1 : 10 µg Human MCF7 breast cancer whole cell lysateLane 2 : 10 µg Human MCF7 breast cancer whole cell lysate, selected for multiple drug resistance with doxorubicin for 72 hoursSecondaryHRP-conjugated Goat anti-Rabbitdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SIRT1 antibody - ab57090 from Abcam

Prices $385.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
ICC/IF image of ab57090 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab57090 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SIRT1 antibody - ab166821 from Abcam

Prices $376.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-SIRT1 antibody (ab166821) at 0.5 µg/ml + Jurkat cell lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-SIRT1 antibody - ab113426 from Abcam

Prices $376.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-SIRT1 antibody (ab113426) at 0.05 µg/ml + Jurkat cell lysate (in RIPA buffer) at 35 µgdeveloped using the ECL technique

Anti-SIRT1 antibody - ab53517 from Abcam

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Anti-SIRT1 antibody (ab53517) at 1 µg/ml + Mouse fetal brain lysate at 35 µg

Anti-SIRT1 antibody - ab138107 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-SIRT1 antibody (ab138107) at 1/500 dilution + COLO205 cell lysate at 30 µg
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