Anti-SIRT1 antibody

• Supplier: Abcam
• Catalog: ab7343
• Prices: $398.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF IP WB
• Species Reactivities: Human
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human SIRT1
• Blocking Peptide: SIRT1 peptide
• Description: Rabbit Polyclonal
• Gene: SIRT1
• Conjugate: Unconjugated

Product images

All lanes : Anti-SIRT1 antibody (ab7343) at 1 µg/mlLane 1 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate - Vector Transfected ControlLane 2 : HEK293 Whole Cell Lysate Transiently Overexpressing SIRT1 Lysates/proteins at 5 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

SIRT1 was immunoprecipitated from HeLa nuclear extract. The  IP'd fraction was incubated with a histone H4 peptide acetylated with tritiated acetyl-coA, in the presence or absence of NAD+ (the cofactor for SIRT's). After incubation the free label (released acetyl groups) was separated from the peptide.

ab7343 (1/200) staining SIRT1 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview

Product References

Suppression of centrosome duplication and amplification by deacetylases. - Suppression of centrosome duplication and amplification by deacetylases.

Ling H, Peng L, Seto E, Fukasawa K. Cell Cycle. 2012 Oct 15;11(20):3779-91.

SIRT1 is a redox-sensitive deacetylase that is post-translationally modified by - SIRT1 is a redox-sensitive deacetylase that is post-translationally modified by

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The SIRT1 activator resveratrol protects SK-N-BE cells from oxidative stress and - The SIRT1 activator resveratrol protects SK-N-BE cells from oxidative stress and

Albani D, Polito L, Batelli S, De Mauro S, Fracasso C, Martelli G, Colombo L, Manzoni C, Salmona M, Caccia S, Negro A, Forloni G. J Neurochem. 2009 Sep;110(5):1445-56.

SIRT1, an antiinflammatory and antiaging protein, is decreased in lungs of - SIRT1, an antiinflammatory and antiaging protein, is decreased in lungs of

Rajendrasozhan S, Yang SR, Kinnula VL, Rahman I. Am J Respir Crit Care Med. 2008 Apr 15;177(8):861-70. doi:

Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via - Sirtuin regulates cigarette smoke-induced proinflammatory mediator release via

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Distinct p53 acetylation cassettes differentially influence gene-expression - Distinct p53 acetylation cassettes differentially influence gene-expression

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Human SirT1 interacts with histone H1 and promotes formation of facultative - Human SirT1 interacts with histone H1 and promotes formation of facultative

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