Anti-SIRT3 antibody
| Name | Anti-SIRT3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab86671 |
| Prices | $394.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-P |
| Species Reactivities | Mouse, Monkey, Hamster |
| Antigen | Synthetic peptide corresponding to Mouse SIRT3 aa 317-330 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Blocking Peptide | SIRT3 peptide |
| Description | Rabbit Polyclonal |
| Gene | Sirt3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-SIRT3 antibody (ab86671) at 1/1000 dilution + Mouse brain tissue lysate at 35 µg/mlSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Anti-SIRT3 antibody (ab86671) at 1/1000 dilution + CHO whole cell lysate at 35 µgSecondaryPeroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinomaa tissue labelling SIRT3 with ab86671. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A Peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.
ICC/IF image of ab86671 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86671, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 rabbit anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
Cellular consequences of oxidative stress in riboflavin responsive multiple - Cellular consequences of oxidative stress in riboflavin responsive multiple
Cornelius N, Corydon TJ, Gregersen N, Olsen RK. Hum Mol Genet. 2014 Aug 15;23(16):4285-301.
Metformin reduces hepatic expression of SIRT3, the mitochondrial deacetylase - Metformin reduces hepatic expression of SIRT3, the mitochondrial deacetylase
Buler M, Aatsinki SM, Izzi V, Hakkola J. PLoS One. 2012;7(11):e49863.
Sirt3 protects in vitro-fertilized mouse preimplantation embryos against - Sirt3 protects in vitro-fertilized mouse preimplantation embryos against
Kawamura Y, Uchijima Y, Horike N, Tonami K, Nishiyama K, Amano T, Asano T, Kurihara Y, Kurihara H. J Clin Invest. 2010 Aug;120(8):2817-28.