Anti-SIRT3 antibody
| Name | Anti-SIRT3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab45067 |
| Prices | $379.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB FC ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human SIRT3 |
| Description | Rabbit Polyclonal |
| Gene | SIRT3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SIRT3 antibody (ab45067) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ICC/IF image of ab45067 stained human HeLa cells. The cells were methanol fixed (5 min), permabilised in PBS-T (20 min) and incubated with the antibody (ab45067, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).