ab68908 staining SIX2 in Mouse Kidney by ICC/IF (Immunocytochemistry/immunofluorescence) 72 hours after Tamoxifen administration to pregnant mice. Cells were fixed with 4% paraformaldehyde and embedded in paraffin. Samples were incubated with primary antibody (1/200) An Alexa Fluor®546-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody. Counter stained with anti- Ac Tubulin and Nuclei stained with DAPI.The number of primary cilia (green) is markedly decreased in both SIX2-positive cells (nephrogenic precursors) and in ureteric cellsKif3aTM kidney (E, asterisk) compared to WT kidney (D). Scale bar= 25 μm
All lanes : Anti-SIX2 antibody (ab68908) at 1 µg/mlLane 1 : Thymus (Human) Tissue Lysate - adult normal tissue (ab30146)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.