Anti-Six5 antibody
| Name | Anti-Six5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab113064 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ICC/IF ICC/IF WB |
| Species Reactivities | Human, Mouse |
| Antigen | Synthetic peptide corresponding to Human Six5 aa 257-286 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH) |
| Description | Rabbit Polyclonal |
| Gene | SIX5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunohistochemistry of formalin fixed and paraffin embedded Human liver tissue labelling Six5 with ab113064.
Immunofluorescence of HeLa cells labelling Six5 with ab113064. Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab113064 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Six5 immunoreactivity is localized to the cytoplasm.
Anti-Six5 antibody (ab113064) at 1/100 dilution + MDA-MB231 cell line lysates at 35 µg