All lanes : Anti-SKA3 antibody (ab175951) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLysates/proteins at 35 µg per lane.
Immunohistochemical analysis of formalin-fixed paraffin-embedded Human testis tissue, labeling SKA3 using ab175951 at a 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometry analysis of HeLa cells labeling SKA3 (green, right histogram), using ab175951 at a 1/10 dilution, and negative control cells (blue, left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.