SKA3

Gene Symbol SKA3
Entrez Gene 221150
Alt Symbol C13orf3, RAMA1
Species Human
Gene Type protein-coding
Description spindle and kinetochore associated complex subunit 3
Other Description spindle and kinetochore-associated protein 3
Swissprots Q5VZV5 A2A331 A2A330 Q8IX90 Q8NBG1 B2RBY2 Q86WR2 Q96D22
Accessions EAX08293 EAX08294 EAX08295 Q8IX90 AF361358 AAO13796 AK090584 BAC03484 AK307976 AK314864 BAG37379 BC013418 AAH13418 BC048988 AAH48988 BI460315 HQ447993 ADQ32474 XM_005266288 XP_005266345 XM_011534994 XP_011533296 NM_001166017 NP_001159489 NM_145061 NP_659498
Function Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it mediates the microtubule-stimulated oligomerization. {ECO:0000269|PubMed:19289083, ECO:0000269|PubMed:19360002}.
Subcellular Location Cytoplasm, cytoskeleton, spindle. Chromosome, centromere, kinetochore. Note=Localizes to the outer kinetochore and spindle microtubules during mitosis in a NDC80 complex-dependent manner.

Ska3 (E-10) - sc-390910 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ska3 (E-10): sc-390910. Western blot analysis of Ska3 expression in non-transfected: sc-117752 (A) and human Ska3 transfected: sc-371164 (B) 293T whole cell lysates.

Ska3 (B-1) - sc-390326 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ska3 (B-1): sc-390326. Western blot analysis of Ska3 expression in non-transfected: sc-117752 (A) and human Ska3 transfected: sc-371164 (B) 293T whole cell lysates.

Ska3 (H-9) - sc-390966 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Ska3 (H-9): sc-390966. Western blot analysis of Ska3 expression in non-transfected: sc-117752 (A) and human Ska3 transfected: sc-371164 (B) 293T whole cell lysates.

Ska3 (H-300) - sc-134939 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Ska3 (H-300): sc-134939. Western blot analysis of Ska3 expression in non-transfected: sc-117752 (A) and human Ska3 transfected: sc-371164 (B) 293T whole cell lysates.

Ska3 (S-13) - sc-84026 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Ska3 (S-13): sc-84026. Western blot analysis of Ska3 expression in non-transfected: sc-117752 (A) and human Ska3 transfected: sc-371164 (B) 293T whole cell lysates.

Ska3 (T-19) - sc-84027 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
Ska3 (T-19): sc-84027. Western blot analysis of Ska3 expression in non-transfected: sc-117752 (A) and human Ska3 transfected: sc-371164 (B) 293T whole cell lysates.

Anti-SKA3 antibody - ab175951 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SKA3 antibody (ab175951) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293 cell lysateLysates/proteins at 35 µg per lane.

Anti-SKA3 antibody - ab91559 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Lane 1 : Anti-SKA3 antibody (ab91559) at 0.5 µg/mlLane 2 : Anti-SKA3 antibody (ab91559) at 1 µg/mlLane 1 : Human testis tissue lysateLane 2 : Human testis tissue lysateLysates/proteins at 15 µg per lane.

Anti-SKA3 antibody - ab118560 from Abcam

Prices $407.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118560 at 5&microg/ml staining SKA3 in Formalin-fixed, Paraffin-embedded Human testis tissue by immunohistochemistry.

Anti-SKA3 antibody - ab134819 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human placenta tissue labelling SKA3 with ab134819 at 3.75µg/ml.

Anti-SKA3 antibody - ab186047 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of SKA3 in immunoprecipitates of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded) using ab186047 at 6 µg/mg lysate for IP (Lane 1). Lane 2 represents rabbit IgG IP control. An alternative anti-SKA3 antibody was used at 1 µg/ml for subsequent western blot detection. Detection: Chemiluminescence with an exposure time of 10 seconds.

Anti-SKA3 antibody - C-terminal - ab186003 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Detection of Human SKA3 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP Jurkat whole cell lysate loaded. ab186003 used for IP at 6µg/ml. For blotting immunoprecipitated SKA3, ab186003 was used at 1 µg/ml.

Anti-RAMA1 / SKA3 Antibody (Internal, Biotin) - LS-C243975 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMA1 / SKA3 Antibody (Internal, AP) - LS-C243973 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMA1 / SKA3 Antibody (Internal, APC) - LS-C243974 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMA1 / SKA3 Antibody (Internal, FITC) - LS-C243976 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMA1 / SKA3 Antibody (Internal, PE) - LS-C243980 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMA1 / SKA3 Antibody (Internal, Azide-free, HRP) - LS-C247498 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMA1 / SKA3 Antibody (aa175-225) - LS-C290858 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMA1 / SKA3 Antibody (Internal) - LS-C327073 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-RAMA1 / SKA3 Antibody (aa362-412) - LS-C290856 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human SKA3 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-SKA3 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Anti-RAMA1 / SKA3 Antibody (aa323-372) - LS-C111654 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
SKA3 antibody LS-C111654 Western blot of ACHN lysate.

Anti-RAMA1 / SKA3 Antibody (aa226-253) - LS-C156969 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
C13orf3 Antibody IHC of formalin-fixed and paraffin-embedded testis tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-RAMA1 / SKA3 Antibody (aa311-323) IHC-plus™ - LS-B7373 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-SKA3 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7373 concentration 3.75 ug/ml.

Anti-RAMA1 / SKA3 Antibody (C-Terminus) IHC-plus™ - LS-B5039 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Anti-SKA3 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B5039 concentration 5 ug/ml.
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