All lanes : Anti-SKAP55 antibody [EPR11360] (ab170882) at 1/1000 dilutionLane 1 : Human thymus lysateLane 2 : Jurkat lysateLane 3 : HuT-78 lysateLane 4 : MOLT4 lysateLysates/proteins at 10 µg per lane.SecondaryHRP labeled goat anti-rabbit IgG at 1/2000 dilution
Immunofluorescent staining of Jurkat cells labeling SKAP55 using ab170882 at a 1/100 dilution (green) and DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized Jurkat cells labeling SKAP55 using ab170882 at a 1/10 dilution (red) or a rabbit IgG (green).