Anti-SKP2 antibody
| Name | Anti-SKP2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab68455 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP WB IHC-P ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | A synthetic peptide corresponding to a sequence at the N terminus of human SKP2, differing from the related rat and mouse sequence by three amino acids |
| Description | Rabbit Polyclonal |
| Gene | SKP2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab68455 at 1µg/ml staining SKP2 in Human mammary cancer tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections). The tissue underwent enzymatic antigen retrieval in pepsin and trypsin. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.
All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/mlLane 1 : Whole cell lysates prepared from Hela cellsLane 2 : Whole cell lysates prepared from 6T-CEM cellsLysates/proteins at 50 µg per lane.SecondaryHRP conjugated Goat anti-rabbit IgG at 1/3000 dilution
All lanes : Anti-SKP2 antibody (ab68455) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
![SKP2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to SKP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab68455.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 53kDa; SKP2](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/494_SKP2-Primary-antibodies-ab68455-6.jpg)
SKP2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to SKP2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab68455.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 53kDa; SKP2
Product References
MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple - MiR-7 triggers cell cycle arrest at the G1/S transition by targeting multiple
Sanchez N, Gallagher M, Lao N, Gallagher C, Clarke C, Doolan P, Aherne S, Blanco A, Meleady P, Clynes M, Barron N. PLoS One. 2013 Jun 6;8(6):e65671.
CDK inhibitor p57 (Kip2) is negatively regulated by COP9 signalosome subunit 6. - CDK inhibitor p57 (Kip2) is negatively regulated by COP9 signalosome subunit 6.
Chen B, Zhao R, Su CH, Linan M, Tseng C, Phan L, Fang L, Yang HY, Yang H, Wang W, Xu X, Jiang N, Cai S, Jin F, Yeung SC, Lee MH. Cell Cycle. 2012 Dec 15;11(24):4633-41.
Transcriptional regulation of S phase kinase-associated protein 2 by NR4A orphan - Transcriptional regulation of S phase kinase-associated protein 2 by NR4A orphan
Gizard F, Zhao Y, Findeisen HM, Qing H, Cohn D, Heywood EB, Jones KL, Nomiyama T, Bruemmer D. J Biol Chem. 2011 Oct 14;286(41):35485-93.