All lanes : Anti-SLC22A7 antibody (ab58683) at 5 µg/mlLane 1 : Molecular weight markerLane 2 : Jurkat cell lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/5000 dilutionObserved band size : 58 kDa (why is the actual band size different from the predicted?)Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human muscle tissue labelling SLC22A7 with ab58683 at 8µg/ml. Arrows indicate positive labelled skeletal muscle cells. Magnification: X400.
All lanes : Anti-SLC22A7 antibody (ab58683) at 2.5 µg/mlLane 1 : Jurkat cell lysateLane 2 : Jurkat cell lysate with blocking peptide at 2 µg/mlLysates/proteins at 25 µg per lane.