All lanes : Anti-SLC6A19 antibody [EPR14154(B)] (ab180516) at 1/10000 dilutionLane 1 : PC3 cell lysateLane 2 : Caco2 cell lysateLane 3 : HeLa cell lysateLane 4 : 293 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat anti-rabbit IgG (H+L) Peroxidase conjugated secondary antibody at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling SLC6A19 with ab180516 at 1/100 dilution. Anti-rabbbit HRP secondary antibody was used and cells counterstained with Hematoxylin.
Immunofluorescent analysis of HeLa cells labeling SLC6A19 with ab180516 at 1/500 dilution. Cells were fixed with 20% Acetone and Goat anti rabbit IgG was used at 1/250 diluton as secondary antibody. Cells were counter-stained with DAPI.