Anti-Slingshot homolog 1 antibody

• Supplier: Abcam
• Catalog: ab76943
• Prices: $390.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IP ICC/IF ICC/IF
• Species Reactivities: Human, Ape, Orangutan
• Antigen: Synthetic peptide corresponding to a region between residues 675 and 725 of human Slingshot homolog 1 (NP_061857
• Description: Rabbit Polyclonal
• Gene: SSH1
• Conjugate: Unconjugated

Product images

All lanes : Anti-Slingshot homolog 1 antibody (ab76943) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µg

ab76943 at 1µg/ml staining Slingshot homolog 1 in HeLa whole cell lysate immunoprecipitated using ab76943 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

ICC/IF image of ab76943 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76943, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

The cofilin phosphatase slingshot homolog 1 (SSH1) links NOD1 signaling to actin - The cofilin phosphatase slingshot homolog 1 (SSH1) links NOD1 signaling to actin

Bielig H, Lautz K, Braun PR, Menning M, Machuy N, Brugmann C, Barisic S, Eisler SA, Andree M, Zurek B, Kashkar H, Sansonetti PJ, Hausser A, Meyer TF, Kufer TA. PLoS Pathog. 2014 Sep 4;10(9):e1004351.

Platelet-derived growth factor (PDGF) regulates Slingshot phosphatase activity - Platelet-derived growth factor (PDGF) regulates Slingshot phosphatase activity

Maheswaranathan M, Gole HK, Fernandez I, Lassegue B, Griendling KK, San Martin A. J Biol Chem. 2011 Oct 14;286(41):35430-7.

ALDH1L1 inhibits cell motility via dephosphorylation of cofilin by PP1 and PP2A. - ALDH1L1 inhibits cell motility via dephosphorylation of cofilin by PP1 and PP2A.

Oleinik NV, Krupenko NI, Krupenko SA. Oncogene. 2010 Nov 25;29(47):6233-44.

The mitotic kinase Aurora-A induces mammary cell migration and breast cancer - The mitotic kinase Aurora-A induces mammary cell migration and breast cancer

Wang LH, Xiang J, Yan M, Zhang Y, Zhao Y, Yue CF, Xu J, Zheng FM, Chen JN, Kang Z, Chen TS, Xing D, Liu Q. Cancer Res. 2010 Nov 15;70(22):9118-28.