Anti-Smad3 (phospho S213) antibody
| Name | Anti-Smad3 (phospho S213) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab63403 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | A synthesized phosphopeptide derived from human Smad3 around the phosphorylation site of serine 213 (PMSPPA) |
| Description | Rabbit Polyclonal |
| Gene | SMAD3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Smad3 (phospho S213) antibody (ab63403) at 1/500 dilutionLane 1 : Extracts from HT29 cellsLane 2 : Extracts from HT29 cells plus phospho peptide
ICC/IF image of ab63403 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab63403 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab63403 staining Smad3 (phospho S213) in Human A549 cells by Immunocytochemistry. Cells were fixed using the HOPE method and blocked for 5 minutes at 25°C. Samples were incubated with primary antibody (1/100) for 1 hour at 25°C. An undiluted HRP-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
Product References
Axl activates autocrine transforming growth factor-beta signaling in - Axl activates autocrine transforming growth factor-beta signaling in
Reichl P, Dengler M, van Zijl F, Huber H, Fuhrlinger G, Reichel C, Sieghart W, Peck-Radosavljevic M, Grubinger M, Mikulits W. Hepatology. 2015 Mar;61(3):930-41.
Serine-204 in the linker region of Smad3 mediates the collagen-I response to - Serine-204 in the linker region of Smad3 mediates the collagen-I response to
Browne JA, Liu X, Schnaper HW, Hayashida T. Exp Cell Res. 2013 Nov 15;319(19):2928-37.