SMAD3

Gene Symbol SMAD3
Entrez Gene 4088
Alt Symbol HSPC193, HsT17436, JV15-2, LDS1C, LDS3, MADH3
Species Human
Gene Type protein-coding
Description SMAD family member 3
Other Description MAD homolog 3|MAD, mothers against decapentaplegic homolog 3|SMA- and MAD-related protein 3|SMAD, mothers against DPP homolog 3|hMAD-3|hSMAD3|mad homolog JV15-2|mad protein homolog|mad3|mothers against DPP homolog 3|mothers against decapentaplegic homolog 3
Swissprots B7Z9Q2 B7Z4Z5 P84022 A8K4B6 B7Z6M9 F5H383
Accessions AAL68976 BAA22032 EAW77788 EAW77789 EAW77790 EAW77791 P84022 AA493306 AB209616 BAD92853 AF151027 AAF36113 AI762628 AI991957 AK026690 AK290881 BAF83570 AK298139 BAH12731 AK300614 BAH13315 AK301348 BAH13461 AK313812 BAG36548 AK316017 BAH14388 AL110265 BC000414 BC007496 BC050743 AAH50743 BE905995 BF058230 BG251847 BM551682 BM722372 BQ917888 BX506233 CR457386 CAG33667 CR749287 CAH18142 DA453132 DA755047 DA977882 EU016552 EU016553 EU446487 ABZ92016 U68019 AAB80960 U76622 AAB18967 XM_011521559 XP_011519861 XM_011521560 XP_011519862 NM_001145102 NP_001138574 NM_001145103 NP_001138575 NM_001145104 NP_001138576 NM_005902 NP_005893
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF- mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. {ECO:0000269|PubMed:10995748, ECO:0000269|PubMed:15241418, ECO:00
Subcellular Location Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1. Co-localizes with LEMD3 at the nucleus inner membrane. MAPK-mediated phosphorylation appears to have no effect on nuclear import. PDPK1 prevents its nuclear translocation in response to TGF-beta.
Top Pathways Cell cycle, Signaling pathways regulating pluripotency of stem cells, Inflammatory bowel disease (IBD), TGF-beta signaling pathway, Adherens junction

Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb - 9520 from Cell Signaling Technology

Prices $109.00, $297.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C25A9
Western blot analysis of extracts from HT-1080, C2C12, or KNRK cells, untreated (-) or treated with TGF-β (10 ng/ml, 30 min; +), using Phospho-Smad3 (Ser423/425) (C25A9) Rabbit mAb (upper) or total Smad3 (C67H9) Rabbit mAb #9523 (lower).

Smad3 Antibody - 9513 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT1080, HeLa and B35 cell lines, using Smad3 Antibody.

Smad3 (C67H9) Rabbit mAb - 9523 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone C67H9
Western blot analysis of extracts from HT1080 (human), C2C12 (mouse) and B35 (rat) using Smad3 (C67H9) Rabbit mAb.

Phospho-Smad3 (Ser423/425) (D12E11) Rabbit mAb - 8769 from Cell Signaling Technology

Host Rabbit
Clonality Monoclonal
Clone D12E11

Smad2/3 (C-8) - sc-133098 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
mad2/3 (C-8): sc-133098. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization.

Smad2/3 (E-1) - sc-376928 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad2/3 (E-1): sc-376928. Western blot analysis of Smad3 expression in non-transfected: sc-117752 (A) and human Smad3 transfected: sc-116400 (B) 293T whole cell lysates.

Smad2/3 (FL-425) - sc-8332 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Smad2/3 (FL-425): sc-8332. Western blot analysis of Smad2 and Smad3 expression in K-562 whole cell lysate.

Smad3 (38-Q) - sc-101154 from Santa Cruz Biotechnology

Prices $295.00
Sizes 50 µg/0.5 ml
Host Mouse
Clonality Monoclonal
Smad3 (38-Q): sc-101154. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human tonsil tissue showing cytoplasmic localization.

p-Smad2/3 (Ser 423/425) - sc-11769 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
p-Smad2/3 (Ser 423/425): sc-11769. Immunoperoxidase staining of human embryonic skin (A) and bone (B) tissues showing nuclear localization. Kindly provided by Dr. Xiao-Mei Cui, University of Southern California.

p-Smad3 (Ser 208) - sc-130218 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
p-Smad3 (Ser 208): sc-130218. Immunoperoxidase staining of formalin fixed, paraffin-embedded human cancer tissue showing cytoplasmic staining.

Anti-Smad3 (phospho S204) antibody - ab63402 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho S204) antibody (ab63402) at 1/500 dilutionLane 1 : Extracts from NIH/3T3 cells, treated with Serum (20%, 15mins)Lane 2 : Extracts from NIH/3T3 cells, treated with Serum (20%, 15mins) plus phospho peptide

Anti-Smad3 (phospho S204) antibody - ab195837 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho S204) antibody (ab195837) at 1/500 dilutionLane 1 : Serum treated NIH-3T3 whole cell lysates with antigen specific peptideLane 2 : Serum treated NIH-3T3 whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Smad3 (phospho S208) antibody - ab192195 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho S208) antibody (ab192195) at 1/500 dilutionLane 1 : Jurkat whole cell lysate with Antigen-specific peptideLane 2 : Jurkat whole cell lysateLysates/proteins at 12.5 µg per lane.developed using the ECL technique

Anti-Smad3 (phospho S208) antibody - ab138659 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-Smad3 (phospho S208) antibody (ab138659) at 1/500 dilution + Jurkat cell lysate at 30 µg

Anti-Smad3 (phospho S213) antibody - ab63403 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho S213) antibody (ab63403) at 1/500 dilutionLane 1 : Extracts from HT29 cellsLane 2 : Extracts from HT29 cells plus phospho peptide

Anti-Smad3 (phospho S423 + S425) antibody - ab51451 from Abcam

Prices $400.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho S423 + S425) antibody (ab51451) at 1/2000 dilutionLane 1 : MEF lysate (control)Lane 2 : MEF lysate, after stimulation with 2 ng/ml of TGF-ß for 1 hourLane 3 : MEF-/-Smad3 lysate (control)Lane 4 : MEF-/-Smad3 lysate, after stimulation with 2 ng/ml of TGF-ß for one hourLane 5 : Hep3B lysate (control)Lane 6 : Hep3B lysate, after stimulation with 2 ng/ml of TGF-ß for one hourLane 7 : HEK 293 lysate (control)

Anti-Smad3 (phospho S423 + S425) antibody - ab118825 from Abcam

Prices $378.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
ab118825, at 2.5 µg/ml, staining Smad3 (phospho S423 + S425) in formalin fixed, paraffin embedded Human skin tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-Smad3 (phospho S423 + S425) antibody [EP823Y] - ab52903 from Abcam

Prices $99.00, $403.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP823Y
ab52903 staining Smad3 in mouse primary embryonic epicardial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100 for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview

Anti-Smad3 (phospho S425) antibody - ab51177 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Ab47439, at dilution of 1/50, staining Smad3 in paraffin embedded human breast carcinoma tissue by Immunohistochemistry. Left image: No peptide. Right image: Phosphopeptide present.

Anti-Smad3 (phospho S425) antibody - ab195908 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho S425) antibody (ab195908) at 1/500 dilutionLane 1 : NIH-3T3 whole cell lysate with antigen specific peptideLane 2 : NIH-3T3 whole cell lysateLysates/proteins at 12.5 µg per lane.

Anti-Smad3 (phospho T179) antibody - ab74062 from Abcam

Prices $379.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho T179) antibody (ab74062) at 1/500 dilutionLane 1 : HeLa cell extracts treated with TNF (20ng/ml, 2mins)Lane 2 : HeLa cell extracts treated with TNF (20ng/ml, 2mins) with immunising peptide at 10 µgLysates/proteins at 30 µg per lane.

Anti-Smad3 (phospho T179) antibody - ab193297 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho T179) antibody (ab193297) at 1/500 dilutionLane 1 : extracts from HeLa cells treated with TNFLane 2 : extracts from HeLa cells treated with TNF with antigen-specific peptide

Anti-Smad3 (phospho T179) antibody - ab195915 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 (phospho T179) antibody (ab195915) at 1/500 dilutionLane 1 : TNF treated HeLa whole cell lysates with antigen specific peptideLane 2 : TNF treated HeLa whole cell lysatesLysates/proteins at 12.5 µg per lane.

Anti-Smad3 antibody - ab84177 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad3 antibody (ab84177) at 1 µg/mlLane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : EGF-treated A431 (Human epithelial carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg/ml per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Smad3 antibody - ab55480 from Abcam

Prices $378.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Overlay histogram showing LoVo cells stained with ab55480 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55480, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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