Anti-Smad3 (phospho S423 + S425) antibody
| Name | Anti-Smad3 (phospho S423 + S425) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab51451 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ELISA ICC/IF ICC/IF |
| Species Reactivities | Mouse, Chicken, Human, Rat, Bovine, Pig, Xenopus, Zebrafish |
| Antigen | Synthetic peptide: PSIRCSSpVSp, corresponding to amino acids 417-425 of Human Smad3 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | SMAD3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Smad3 (phospho S423 + S425) antibody (ab51451) at 1/2000 dilutionLane 1 : MEF lysate (control)Lane 2 : MEF lysate, after stimulation with 2 ng/ml of TGF-ß for 1 hourLane 3 : MEF-/-Smad3 lysate (control)Lane 4 : MEF-/-Smad3 lysate, after stimulation with 2 ng/ml of TGF-ß for one hourLane 5 : Hep3B lysate (control)Lane 6 : Hep3B lysate, after stimulation with 2 ng/ml of TGF-ß for one hourLane 7 : HEK 293 lysate (control)
ab51451 at 2.5 µg/ml staining epidermal keratinocytes at 40X. This image shows strong nuclear staining with localization of the antibody as the precipated red siganl and a hematoxylin purple nuclear counterstain.
ab51451 staining Smad3 (phospho S423 + S425) in PC3 and LnCAP cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 70% ethanol and blocked for 1 hour at 26°C. Samples were incubated with primary antibody (1/150) for 16 hours at 4°C. An IRDye® 800CW-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. NC-PC3 - negative control PC3 cells, NC-LnCAP - negative control LnCAP cells.See Abreview
Product References
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