Anti-Smad3 (phospho S423 + S425) antibody [EP823Y]

• Supplier: Abcam
• Catalog: ab52903
• Prices: $99.00, $403.00
• Sizes: 10 µl, 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP823Y
• Applications: WB ICC/IF ICC/IF ELISA IHC-F IHC-P
• Species Reactivities: Mouse, Human, Drosophila
• Antigen: A synthetic phospho specific peptide corresponding to residues surrounding Ser423 and Ser425 of human Smad3
• Blocking Peptide: Smad3 (phospho S423 + S425) peptide
• Description: Rabbit Monoclonal
• Gene: SMAD3
• Conjugate: Unconjugated

Product images

ab52903 staining Smad3 in mouse primary embryonic epicardial cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% formaldehyde, permeabilized with 0.5% Triton X-100 and blocked with PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100 for 1 hour at 20°C. Samples were incubated with primary antibody (1/100 in PBS + 1% BSA + 10% goat serum + 0.1% Triton X-100) for 16 hours at 4°C. An Alexa Fluor®488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview

All lanes : Anti-Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903) at 1/2000 dilutionLane 1 : (A) HL-60 cell lysates at 10µg untreatedLane 2 : (B) HL-60 cell lysates at 10µg treated with TGF.

Immunofluorescent staining of Smad3 in HeLa cells using ab52903 at 1/100 dilution.

Immunohistochemical analysis of Smad3 in paraffin embedded human liver carcinoma tissue using ab52903 at 1/100 dilution.Immunohistochemical analysis of Smad3 in paraffin embedded human liver carcinoma tissue using ab52903 at 1/100 dilution.

All lanes : Anti-Smad3 (phospho S423 + S425) antibody [EP823Y] (ab52903) at 1/1000 dilutionLane 1 : Lysate prepared from untreated human A549 cellsLane 2 : Lysate prepared from untreated human A549 cells for 30minLane 3 : Lysate prepared from TGF-ß1 cells at 10ng/ml for 30minLane 4 : Lysate prepared from TNF-a cells at 20ng/ml for 30minLane 5 : Lysate prepared from TGF-ß1 and TNF-a cells at above doses for 30minLane 6 : Blank DMEM mediaLysates/proteins at 20 µg per lane.SecondaryDonkey Anti-Rabbit IgG H&L (HRP) (ab16284)developed using the ECL techniquePerformed under reducing conditions.

ab52903 staining Smad3 (phospho S423 + S425) in human TII Pneumocyte A549c ells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton x100 before blocking with 3% BSA for 1 hour at RT. Samples were incubated with primary antibody (1/200: in 3% BSA in 1x PBST) for 24 hours at 4°C. A TRITC-conjugated goat polyclonal to rabbit IgG was used as secondary antibody at 1/200 dilution.See Abreview

Product References

Attenuation of TGF-beta signaling supports tumor progression of a - Attenuation of TGF-beta signaling supports tumor progression of a

Biswas T, Gu X, Yang J, Ellies LG, Sun LZ. Cancer Lett. 2014 Apr 28;346(1):129-38.

Vitamin D receptor regulates TGF-beta signalling in systemic sclerosis. - Vitamin D receptor regulates TGF-beta signalling in systemic sclerosis.

Zerr P, Vollath S, Palumbo-Zerr K, Tomcik M, Huang J, Distler A, Beyer C, Dees C, Gela K, Distler O, Schett G, Distler JH. Ann Rheum Dis. 2015 Mar;74(3):e20.

Postsynaptic glutamate receptors regulate local BMP signaling at the Drosophila - Postsynaptic glutamate receptors regulate local BMP signaling at the Drosophila

Sulkowski M, Kim YJ, Serpe M. Development. 2014 Jan;141(2):436-47.

Loss of epithelial p53 and alphav integrin cooperate through Akt to induce - Loss of epithelial p53 and alphav integrin cooperate through Akt to induce

Savar A, Acin S, Gonzalez CL, El-Sawy T, Mejia O, Li Z, Esmaeli B, Lacy-Hulbert A, El-Naggar AK, McCarty JH, Caulin C. Oncogene. 2015 Jan 22;34(4):516-24.

GSK3 inhibitors regulate MYCN mRNA levels and reduce neuroblastoma cell viability - GSK3 inhibitors regulate MYCN mRNA levels and reduce neuroblastoma cell viability

Duffy DJ, Krstic A, Schwarzl T, Higgins DG, Kolch W. Mol Cancer Ther. 2014 Feb;13(2):454-67.

Soluble MMP-14 produced by bone marrow-derived stromal cells sheds epithelial - Soluble MMP-14 produced by bone marrow-derived stromal cells sheds epithelial

Tobar N, Avalos MC, Mendez N, Smith PC, Bernabeu C, Quintanilla M, Martinez J. Carcinogenesis. 2014 Aug;35(8):1770-9.

Piwi is required in multiple cell types to control germline stem cell lineage - Piwi is required in multiple cell types to control germline stem cell lineage

Ma X, Wang S, Do T, Song X, Inaba M, Nishimoto Y, Liu LP, Gao Y, Mao Y, Li H, McDowell W, Park J, Malanowski K, Peak A, Perera A, Li H, Gaudenz K, Haug J, Yamashita Y, Lin H, Ni JQ, Xie T. PLoS One. 2014 Mar 21;9(3):e90267.

Interleukin-6 (IL-6) trans signaling drives a STAT3-dependent pathway that leads - Interleukin-6 (IL-6) trans signaling drives a STAT3-dependent pathway that leads

O'Reilly S, Ciechomska M, Cant R, van Laar JM. J Biol Chem. 2014 Apr 4;289(14):9952-60.

Wild-type and mutant p53 differentially regulate NADPH oxidase 4 in - Wild-type and mutant p53 differentially regulate NADPH oxidase 4 in

Boudreau HE, Casterline BW, Burke DJ, Leto TL. Br J Cancer. 2014 May 13;110(10):2569-82.

Growth control by a moving morphogen gradient during Drosophila eye development. - Growth control by a moving morphogen gradient during Drosophila eye development.

Wartlick O, Julicher F, Gonzalez-Gaitan M. Development. 2014 May;141(9):1884-93.