Anti-Smad4 antibody [EP618Y] (Alexa Fluor® 647)
| Name | Anti-Smad4 antibody [EP618Y] (Alexa Fluor® 647) |
|---|---|
| Supplier | Abcam |
| Catalog | ab196187 |
| Prices | $431.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP618Y |
| Applications | ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Smad4 aa 500 to the C-terminus (C terminal) |
| Description | Rabbit Monoclonal |
| Gene | SMAD4 |
| Conjugate | Alexa Fluor® 647 |
| Supplier Page | Shop |
Product images
![ab196187 staining Smad4 in A549 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196187 at 1/100 Dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/1946_ab196187-234495-ab196187-ap2177740-5ug-ab195887-A549p.jpg)
ab196187 staining Smad4 in A549 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196187 at 1/100 Dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).