SMAD4

Gene Symbol SMAD4
Entrez Gene 4089
Alt Symbol DPC4, JIP, MADH4, MYHRS
Species Human
Gene Type protein-coding
Description SMAD family member 4
Other Description MAD homolog 4|SMAD, mothers against DPP homolog 4|deleted in pancreatic carcinoma locus 4|deletion target in pancreatic carcinoma 4|mothers against decapentaplegic homolog 4|mothers against decapentaplegic, Drosophila, homolog of, 4
Swissprots A8K405 Q13485
Accessions AAC03051 AHA34184 AHA34185 AHA34186 BAB40977 EAW62985 EAW62986 EAW62987 Q13485 AK290770 BAF83459 AU120224 BC002379 AAH02379 BM701399 BX647129 DQ891840 ABM82766 DQ895028 ABM85954 U44378 AAA91041 NM_005359 NP_005350
Function In muscle physiology, plays a central role in the balance between atrophy and hypertrophy. When recruited by MSTN, promotes atrophy response via phosphorylated SMAD2/4. MSTN decrease causes SMAD4 release and subsequent recruitment by the BMP pathway to promote hypertrophy via phosphorylated SMAD1/5/8. Acts synergistically with SMAD1 and YY1 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression. Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions (By similarity). Common SMAD (co-SMAD) is the coactivator and mediator of signal transduction by TGF-beta (transforming growth factor). Component of the heterotrimeric SMAD2/SMAD3-SMAD4 complex that forms in the nucleus and is required for the TGF-mediated signaling. Promotes binding of the SMAD2/SMAD4/FAST-1 complex to DNA and provides an activation function required for SMAD1 or SMAD2 to stimulate transcription. Component of the multimeric SMAD3/SMAD
Subcellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with R- SMAD. PDPK1 prevents its nuclear translocation in response to TGF- beta.
Top Pathways Wnt signaling pathway, Pancreatic cancer, HTLV-I infection, Colorectal cancer, Chronic myeloid leukemia

Smad4 Antibody - 9515 from Cell Signaling Technology

Prices $129.00, $239.00
Sizes 40 µl (4 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from COS, NIH3T3, PC12, and SK-N-MC cells, using Smad4 Antibody.

Smad4 (B-8) - sc-7966 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad4 (B-8): sc-7966. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining (A). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast tumor showing cytoplasmic and nuclear staining (B).

Smad4 (C-20) - sc-1909 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad4 (C-20): sc-1909. Western blot analysis of Smad4 expression in Ramos whole cell lysate.

Smad4 (H-552) - sc-7154 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Smad4 (H-552): sc-7154. Western blot analysis of Smad4 expression in Hep G2 (A) and NIH/3T3 (B) whole cell lysates.

Smad4 (N-16) - sc-1908 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad4 (N-16): sc-1908. Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic localization.

Smad4 (SMD46) - sc-56775 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad4 (SMD46): sc-56775. Western blot analysis of Smad4 expression in Ramos whole cell lysate.

Smad4 (SPM448) - sc-56479 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad4 (SPM448): sc-56479. Western blot analysis of Smad4 expression in Ramos whole cell lysate.

Smad4 (DCS-46) - sc-73599 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad4 (DCS-46): sc-73599. Western blot analysis of Smad4 expression in Ramos whole cell lysate.

Smad4 (0.N.557) - sc-73040 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
Smad4 (0.N.557): sc-73040. Western blot analysis of Smad4 expression in Ramos whole cell lysate.

Anti-Smad4 antibody - ab182637 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smad4 antibody (ab182637) at 1/300 dilutionLane 1 : Human liver cancer lysateLane 2 : HeLa cell lysateLysates/proteins at 30 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution

Anti-Smad4 antibody [4G1C6] - ab130242 from Abcam

Prices $385.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4G1C6
ab130242, at 1/200 dilution, staining Smad4 in paraffin embedded Human lung cancer tissue by Immunohistochemistry. Detected using DAB staining.

Anti-Smad4 antibody [EP618Y] - ab40759 from Abcam

Prices $403.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP618Y
Anti-Smad4 antibody [EP618Y] (ab40759) at 1/5000 dilution (unpurified) + SHSY5Y cell lysate at 10 µg

Anti-Smad4 antibody [EP618Y] (Alexa Fluor® 647) - ab196187 from Abcam

Prices $431.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP618Y
ab196187 staining Smad4 in A549 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized in 0.1% Triton X-100 for 5 minutes and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab196187 at 1/100 Dilution (shown in red) and ab195887, Mouse monoclonal [DM1A] to alpha Tubulin (Alexa Fluor® 488, shown in green) at 2µg/ml overnight at +4°C. Nuclear DNA was labelled in blue with DAPI.Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Anti-Smad4 antibody [EP618Y] (HRP) - ab195554 from Abcam

Prices $425.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP618Y
Anti-Smad4 antibody [EP618Y] (HRP) (ab195554) at 1/5000 dilution + SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µgdeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Smad4 antibody [SMD46 (same as DCS-46)] - ChIP Grade - ab3219 from Abcam

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone SMD46 (same as DCS-46)
The image is of a colony of undifferentiated human embryonic stem cells (H9). The cells are positive for Smad4 with staining localised in the nucleus. Dilution: 1/50

Smad4 Antibody (EP618Y) - NBP1-44389 from Novus Biologicals

Host Rabbit
Clonality Monoclonal
Clone EP618Y

Smad4 Antibody - NBP1-84992 from Novus Biologicals

Host Rabbit
Clonality Polyclonal
Western Blot: SMAD4 Antibody [NBP1-84992] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells).

Smad4 Antibody - NBP2-24951 from Novus Biologicals

Prices $99.00, $229.00
Sizes 25 µg, 100 µg
Host Rabbit
Clonality Polyclonal

Smad4 Antibody [Unconjugated] - AF2097 from Novus Biologicals

Prices $99.00, $369.00
Sizes 25 µg, 100 µg
Host Goat
Clonality Polyclonal

Smad4 Antibody (4G1C6) - NBP1-51646 from Novus Biologicals

Prices $349.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 4G1C6
Western Blot: Smad4 Antibody (4G1C6) [NBP1-51646] - Analysis using SMAD4 mouse mAb against A431 (1), SK-N-SH (2), K562 (3), HepG2 (4) and HUVE12 (5) cell lysate.

Smad4 Antibody (253327) - MAB20971 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 253327
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and HEK293 human embryonic kidney cell line. PVDF membrane was probed with 0.5 µg/mL of Human Smad4 Monoclonal Antibody (Catalog # MAB20971) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Smad4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Smad4 Antibody (253343) [Unconjugated] - MAB2097 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 253343
HeLa human cell line was stained with Mouse Anti-Human Smad4 Monoclonal Antibody (Catalog # MAB2097, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Smad4 Antibody (253343) [Phycoerythrin] - IC2097P from Novus Biologicals

Prices $299.00
Sizes 100 tests
Host Mouse
Clonality Monoclonal
Clone 253343
HeLa human cervical epithelial carcinoma cell line was stained with Mouse Anti-Human Smad4 PE-conjugated Monoclonal Antibody (Catalog # IC2097P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

Smad4 Antibody [Biotin] - BAF2097 from Novus Biologicals

Prices $390.00
Sizes 50 µg
Host Goat
Clonality Polyclonal

ANTI-SMAD4 antibody produced in mouse - SAB1412597 from Sigma-Aldrich

Prices $347.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 2E7
<B>Immunoblotting</B><BR/>Monoclonal Anti-SMAD4: <B>Cat. No. SAB1412597</B>: Antibody concentration: 1 μg/mL. Specific band of ~37 kDa using immunogen protein lysate.
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