Anti-SMAD5 antibody
| Name | Anti-SMAD5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab13724 |
| Prices | $394.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Zebrafish |
| Antigen | Synthetic peptides, corresponding to amino acids 20-34 (GWKQGDEEEKWAEKA) and 316-330 (VNRNSTIENTRRHIG) of Human SMAD 5 |
| Description | Rabbit Polyclonal |
| Gene | SMAD5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab13724 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13724, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-SMAD5 antibody (ab13724) at 1/500 dilutionLane 1 : Whole embryo lysates prepared from Zebrafish as control morpholinos.Lane 2 : Whole embryo lysates prepared from Zebrafish embryos injected with ints5 morpholinos.
IHC image of SMAD5 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13724, 1/200 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
The Integrator subunits function in hematopoiesis by modulating Smad/BMP - The Integrator subunits function in hematopoiesis by modulating Smad/BMP
Tao S, Cai Y, Sampath K. Development. 2009 Aug;136(16):2757-65.
The Integrator subunits function in hematopoiesis by modulating Smad/BMP - The Integrator subunits function in hematopoiesis by modulating Smad/BMP
Tao S, Cai Y, Sampath K. Development. 2009 Aug;136(16):2757-65.