SMAD5

Gene Symbol SMAD5
Entrez Gene 4090
Alt Symbol DWFC, JV5-1, MADH5
Species Human
Gene Type protein-coding
Description SMAD family member 5
Other Description MAD, mothers against decapentaplegic homolog 5|SMA- and MAD-related protein 5|SMAD, mothers against DPP homolog 5|mothers against decapentaplegic homolog 5|mothers against decapentaplegic, drosophila, homolog of, 5
Swissprots Q15798 Q99717 Q9UQA1 O14688
Accessions AAB82655 AAB92396 EAW62194 EAW62195 EAW62196 Q99717 AF009678 AAB72180 AF009745 AF010601 AAB66353 AF071106 AAD20799 AF071107 AAD20800 AF071108 AAD20801 AF071109 AAD20802 AF071110 AAD20803 AK001036 AK055211 AK311231 AK313397 BAG36195 BC009682 AAH09682 BK001394 BX510058 BX646699 BX648328 CR457424 CAG33705 CR749366 CAH18219 CR749473 CAH18303 HQ448272 ADQ32752 U59913 AAC50791 U73825 AAB95090 NM_001001419 NP_001001419 NM_001001420 NP_001001420 NM_005903 NP_005894
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Subcellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
Tissue Specificity Ubiquitous.
Top Pathways TGF-beta signaling pathway, Signaling pathways regulating pluripotency of stem cells

Smad5 Antibody - 9517 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from SK-N-MC, COS and PC12 cells, using Smad5 Antibody.

Smad5 (D4G2) Rabbit mAb - 12534 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D4G2
Western blot analysis of extracts from various cell lines using Smad5 (D4G2) Rabbit mAb.

Smad5 (Q-20) - sc-26418 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad5 (Q-20): sc-26418. Western blot analysis of Smad5 expression in non-transfected: sc-117750 (A) and human Smad5 transfected: sc-111365 (B) whole cell lysates.

Smad5 (T-13) - sc-26419 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Smad5 (D-20) - sc-7443 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad5 (D-20): sc-7443. Western blot analysis of Smad5 expression in Sol8 (A) and C2C12 (B) whole cell lysates.

Smad5 (YY-6) - sc-101151 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
Smad5 (YY-6): sc-101151. Western blot analysis of Smad5 expression in HeLa nuclear extract.

Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] - ab76296 from Abcam

Prices $381.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP728(2)AY
All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [EP728(2)AY] (ab76296) at 1/500 dilutionLane 1 : HeLa cell lysate - untreatedLane 2 : HeLa cell lysate - treated with BMP-4Lysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] - ab92698 from Abcam

Prices $388.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone MMC-1-104-3
All lanes : Anti-SMAD5 (phospho S463 + S465) antibody [MMC-1-104-3] (ab92698) at 1/500 dilutionLane 1 : HeLa cell lysate (untreated)Lane 2 : HeLa cell lysate (treated with BMP-4)Lysates/proteins at 10 µg per lane.SecondaryHRP labelled Goat anti-Rabbit antibody at 1/2000 dilution

Anti-SMAD5 antibody - ab88559 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SMAD5 antibody (ab88559) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-SMAD5 antibody - ab55484 from Abcam

Prices $376.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-SMAD5 antibody - ab156100 from Abcam

Prices $376.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-SMAD5 antibody (ab156100) at 1/250 dilutionLane 1 : Hela cell line lysate with no blocking peptideLane 2 : Hela cell line lysate with blocking peptideLysates/proteins at 12.5 µg per lane.

Anti-SMAD5 antibody - ab13724 from Abcam

Prices $394.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
ICC/IF image of ab13724 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13724, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SMAD5 antibody [3H9] - ab110022 from Abcam

Host Mouse
Clonality Monoclonal
Clone 3H9
All lanes : Anti-SMAD5 antibody [3H9] (ab110022) at 1/500 dilutionLane 1 : Hela cell lysate. Lane 2 : SK-N-SH cell lysate. Lane 3 : PC-12 cell lysate. Lane 4 : Jurkat cell lysate. Lane 5 : K562 cell lysate.

Anti-SMAD5 antibody [4B10-B10-B6] - ab151267 from Abcam

Prices $384.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 4B10-B10-B6
Anti-SMAD5 antibody [4B10-B10-B6] (ab151267) at 1/1000 dilution + Mouse testis lysate

Anti-SMAD5 antibody [EP619Y] - ab40771 from Abcam

Prices $390.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP619Y
Anti-SMAD5 antibody [EP619Y] (ab40771) at 1/1000 dilution + Cos-1 cell lysate at 10 µg

Anti-SMAD5 Antibody - LS-C48446 from LifeSpan Bioscience

Prices $445.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMAD5 Antibody (aa20-34) - LS-C10229 from LifeSpan Bioscience

Prices $415.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD5 Antibody (aa241-269, APC) - LS-C269627 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD5 Antibody (aa241-269, AP) - LS-C269625 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD5 Antibody (aa241-269, Biotin) - LS-C269628 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD5 Antibody (aa241-269, FITC) - LS-C269629 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD5 Antibody (aa241-269, Azide-free, HRP) - LS-C269630 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD5 Antibody (aa241-269, PE) - LS-C269631 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD5 Antibody (MH2 Domain, clone EP619Y) - LS-C49650 from LifeSpan Bioscience

Prices $490.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EP619Y
Western blot analysis on Cos-1 cell lysate using anti-Smad 5 antibody, dilution 1:1000.

Anti-SMAD5 Antibody (aa193-242) - LS-C29765 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
Raji Cell Lysate
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