Anti-SMAD9 antibody
| Name | Anti-SMAD9 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab124094 |
| Prices | $370.00 |
| Sizes | 400 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 199-228 of Human SMAD9 (NP_005896 |
| Description | Rabbit Polyclonal |
| Gene | SMAD9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence of MCF-7 cells labelling SMAD9 with ab124094. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab124094 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SMAD9 immunoreactivity is localized to the cytoplasm and nucleus.
Anti-SMAD9 antibody (ab124094) at 1/100 dilution + A549 cell line lysates at 35 µg