SMAD9

Gene Symbol SMAD9
Entrez Gene 4093
Alt Symbol MADH6, MADH9, PPH2, SMAD8, SMAD8A, SMAD8B
Species Human
Gene Type protein-coding
Description SMAD family member 9
Other Description MAD homolog 9|Mothers against decapentaplegic, drosophila, homolog of, 9|SMAD, mothers against DPP homolog 9|mothers against decapentaplegic homolog 9
Swissprots A2A2Y6 O15198 O14989 Q5TBA1
Accessions EAX08570 EAX08571 EAX08572 EAX08573 O15198 AK311686 AK314920 AW191604 BC011559 AAH11559 BC067766 BC104760 AAI04761 BC104762 AAI04763 BC143240 AAI43241 BC143256 CN291481 D83760 BAA21128 D83761 BAA21129 DB501041 EU446860 ABZ92389 EU831977 ACE87418 EU832070 ACE86732 XM_005266401 XP_005266458 XM_005266403 XP_005266460 XM_005266404 XP_005266461 XM_006719827 XP_006719890 XM_011535096 XP_011533398 NM_001127217 NP_001120689 NM_005905 NP_005896
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD9 is a receptor-regulated SMAD (R-SMAD).
Subcellular Location Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}. Note=In the cytoplasm in the absence of ligand. Migration to the nucleus when complexed with SMAD4 (By similarity). {ECO:0000250}.
Tissue Specificity Expressed in heart, brain, placenta, lung, skeletal muscle, prostate, testis, ovary and small intestine. Also expressed in fetal brain, lung and kidney.
Top Pathways TGF-beta signaling pathway, Signaling pathways regulating pluripotency of stem cells

Smad8 (E-19) - sc-30733 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Smad8 (E-19): sc-30733. Western blot analysis of Smad8 expression in non-transfected: sc-117752 (A) and human Smad8 transfected: sc-177951 (B) 293T whole cell lysates.

Smad8 (A-17) - sc-30732 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Smad8 (R-64) - sc-11393 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Smad8 (R-64): sc-11393. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubuli at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.

Smad8 (S-20) - sc-7442 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Smad8 (V-20) - sc-30734 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-SMAD9 antibody - ab115900 from Abcam

Prices $407.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
ab115900, at 5 µg/ml, staining SMAD9 in formalin fixed, paraffin embedded Human kidney by Immunohistochemistry. Developed using biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-SMAD9 antibody - ab48011 from Abcam

Prices $370.00
Sizes 200 µl
Host Goat
Clonality Polyclonal
Anti-SMAD9 antibody (ab48011) at 0.3 µg/ml + Human Skin lysate in RIPA buffer at 35 µgdeveloped using the ECL technique

Anti-SMAD9 antibody - ab96698 from Abcam

Prices $370.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal
Anti-SMAD9 antibody (ab96698) at 1/500 dilution + NT2D1 whole cell lysate at 30 µg

Anti-SMAD9 antibody - ab124094 from Abcam

Prices $370.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of MCF-7 cells labelling SMAD9 with ab124094. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab124094 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). SMAD9 immunoreactivity is localized to the cytoplasm and nucleus.

Anti-SMAD9 Antibody (aa166-430) - LS-C186364 from LifeSpan Bioscience

Prices $335.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunofluorescence of paraformaldehyde-fixed HeLa using SMAD9 antibody at 1:500 dilution.

Anti-SMAD9 Antibody (aa166-179) IHC-plus™ - LS-B2646 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-SMAD9 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2646 concentration 5 ug/ml.

Anti-SMAD9 Antibody (aa18-32) - LS-C39136 from LifeSpan Bioscience

Prices $415.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMAD9 Antibody (aa158-223) - LS-C182951 from LifeSpan Bioscience

Prices $650.00
Sizes 100 µg
Host Sheep
Clonality Polyclonal

Anti-SMAD9 Antibody (clone 3E5) - LS-C197638 from LifeSpan Bioscience

Prices $355.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 3E5

Anti-SMAD9 Antibody (aa200-228, AP) - LS-C269643 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD9 Antibody (aa200-228, APC) - LS-C269644 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD9 Antibody (aa200-228, Biotin) - LS-C269646 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD9 Antibody (aa200-228, FITC) - LS-C269647 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD9 Antibody (aa200-228, Azide-free, HRP) - LS-C269648 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD9 Antibody (aa200-228, PE) - LS-C269650 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD9 Antibody (aa199-228) - LS-C325192 from LifeSpan Bioscience

Prices $435.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMAD9 Antibody (Internal, clone EPR5023(2)) - LS-C138862 from LifeSpan Bioscience

Prices $395.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR5023(2)
Western blot analysis on (A) Saos-2, (B) 293T, (C) human skin, and (D) A549 lysates using anti-Smad9 antibody.

Anti-SMAD9 Antibody (aa226-459) - LS-C314902 from LifeSpan Bioscience

Prices $405.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of recombinant SMAD9.

Anti-SMAD9 Antibody (aa200-228) - LS-C161303 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Fluorescent confocal image of MCF-7 cell stained with SMAD9 Antibody. MCF-7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with SMAD9 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). SMAD9 immunoreactivity is localized to cytoplasm and nucleus significantly.

Smad9 Antibody - AF7215 from Novus Biologicals

Prices $99.00, $359.00
Sizes 25 µg, 100 µg
Host Sheep
Clonality Polyclonal
Smad9 was detected in immersion fixed paraffin-embedded sections of human prostate cancer tissue using Sheep Anti-Human Smad9 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7215) at 3 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to nuclei in glandular epithelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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