Anti-SMARCA2 / BRM antibody - ChIP Grade
| Name | Anti-SMARCA2 / BRM antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab15597 |
| Prices | $403.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IP ICC/IF ICC/IF IHC-F ChIP WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Mouse BRM/ SMARCA2 fusion protein derived from within residues 48-214 of the corresponding human sequence |
| Description | Rabbit Polyclonal |
| Gene | SMARCA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
A) ab15597 specifically immunoprecipitates Brm. Immunoprecipitations were performed on bacculovirus–expressed Flag-tagged Brm and Brg1 from insect cells. 2µg of ab15597 (left hand side) and 2µg of J1 (right hand side) were used for each IP. J1 is a polyclonal antibody recognising both, Brg1 and Brm.B) ab15597 chromatin immunoprecipitates Brm. Two cell lines 1 and 2 were treated with different stimuli and subjected to the ChIP procedure. Cells were fixed with formaldehyde for 10mins. The ChIP was performed with 2µg of 15597 or J1 and 10µl of protein G Dynal beads. The immunoprecipitated DNA was quanified by real-time PCR with primers specific for genes A and B, respectively. J1 is a polyclonal antibody recognising both, Brg1 and Brm.
Immunofluorescence using the Brm antibody ab15597.It appears the antibody works well in IF detecting BRM in D98oR WT cells. The D98oR BB10 blank omitted the primary antibody as a control. However, it was also positive on a cell line that has Brm knocked down by RNAi, (the BB10 cell line). This may be because RNAi is simply a knock down not a knock out or could be that the antibody is so good it is picking up the small amount of Brm remaining in the BB10s (highly possible), or the dilution is too high. Or, it may be that its crossreacting with another protein (though there was no cross rectivity with Brg1 in WB). Original magnification 400x. ab15597 dilution 1:100. Work is continuing on characterising this ab in IF.
All lanes : Anti-SMARCA2 / BRM antibody - ChIP Grade (ab15597) at 1 µg/mlLane 1 : D98oR (subclone HeLa) positive control lysateLane 2 : SW13 (Human adrenal adenocarcinoma) negative control lysateLysates/proteins at 20 µg per lane.
All lanes : Anti-SMARCA2 / BRM antibody - ChIP Grade (ab15597) at 1 µg/mlLane 1 : Brain (Mouse) Tissue LysateLane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLysates/proteins at 20 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Product References
Germline and somatic SMARCA4 mutations characterize small cell carcinoma of the - Germline and somatic SMARCA4 mutations characterize small cell carcinoma of the
Witkowski L, Carrot-Zhang J, Albrecht S, Fahiminiya S, Hamel N, Tomiak E, Grynspan D, Saloustros E, Nadaf J, Rivera B, Gilpin C, Castellsague E, Silva-Smith R, Plourde F, Wu M, Saskin A, Arseneault M, Karabakhtsian RG, Reilly EA, Ueland FR, Margiolaki A, Pavlakis K, Castellino SM, Lamovec J, Mackay HJ, Roth LM, Ulbright TM, Bender TA, Georgoulias V, Longy M, Berchuck A, Tischkowitz M, Nagel I, Siebert R, Stewart CJ, Arseneau J, McCluggage WG, Clarke BA, Riazalhosseini Y, Hasselblatt M, Majewski J, Foulkes WD. Nat Genet. 2014 May;46(5):438-43.
MITF-independent pro-survival role of BRG1-containing SWI/SNF complex in melanoma - MITF-independent pro-survival role of BRG1-containing SWI/SNF complex in melanoma
Ondrusova L, Vachtenheim J, Reda J, Zakova P, Benkova K. PLoS One. 2013;8(1):e54110.
S-nitrosylation of HDAC2 regulates the expression of the chromatin-remodeling - S-nitrosylation of HDAC2 regulates the expression of the chromatin-remodeling
Nott A, Nitarska J, Veenvliet JV, Schacke S, Derijck AA, Sirko P, Muchardt C, Pasterkamp RJ, Smidt MP, Riccio A. Proc Natl Acad Sci U S A. 2013 Feb 19;110(8):3113-8. doi:
BRG1 promotes survival of UV-irradiated melanoma cells by cooperating with MITF - BRG1 promotes survival of UV-irradiated melanoma cells by cooperating with MITF
Saladi SV, Wong PG, Trivedi AR, Marathe HG, Keenen B, Aras S, Liew ZQ, Setaluri V, de la Serna IL. Pigment Cell Melanoma Res. 2013 May;26(3):377-91.
Oncogenic targeting of BRM drives malignancy through C/EBPbeta-dependent - Oncogenic targeting of BRM drives malignancy through C/EBPbeta-dependent
Damiano L, Stewart KM, Cohet N, Mouw JK, Lakins JN, Debnath J, Reisman D, Nickerson JA, Imbalzano AN, Weaver VM. Oncogene. 2014 May 8;33(19):2441-53.
SWI/SNF chromatin-remodeling factor Smarcd3/Baf60c controls - SWI/SNF chromatin-remodeling factor Smarcd3/Baf60c controls
Jordan NV, Prat A, Abell AN, Zawistowski JS, Sciaky N, Karginova OA, Zhou B, Golitz BT, Perou CM, Johnson GL. Mol Cell Biol. 2013 Aug;33(15):3011-25.
ARID1a-DNA interactions are required for promoter occupancy by SWI/SNF. - ARID1a-DNA interactions are required for promoter occupancy by SWI/SNF.
Chandler RL, Brennan J, Schisler JC, Serber D, Patterson C, Magnuson T. Mol Cell Biol. 2013 Jan;33(2):265-80.
Heterozygous missense mutations in SMARCA2 cause Nicolaides-Baraitser syndrome. - Heterozygous missense mutations in SMARCA2 cause Nicolaides-Baraitser syndrome.
Van Houdt JK, Nowakowska BA, Sousa SB, van Schaik BD, Seuntjens E, Avonce N, Sifrim A, Abdul-Rahman OA, van den Boogaard MJ, Bottani A, Castori M, Cormier-Daire V, Deardorff MA, Filges I, Fryer A, Fryns JP, Gana S, Garavelli L, Gillessen-Kaesbach G, Hall BD, Horn D, Huylebroeck D, Klapecki J, Krajewska-Walasek M, Kuechler A, Lines MA, Maas S, Macdermot KD, McKee S, Magee A, de Man SA, Moreau Y, Morice-Picard F, Obersztyn E, Pilch J, Rosser E, Shannon N, Stolte-Dijkstra I, Van Dijck P, Vilain C, Vogels A, Wakeling E, Wieczorek D, Wilson L, Zuffardi O, van Kampen AH, Devriendt K, Hennekam R, Vermeesch JR. Nat Genet. 2012 Feb 26;44(4):445-9, S1.
SS18 together with animal-specific factors defines human BAF-type SWI/SNF - SS18 together with animal-specific factors defines human BAF-type SWI/SNF
Middeljans E, Wan X, Jansen PW, Sharma V, Stunnenberg HG, Logie C. PLoS One. 2012;7(3):e33834.
Diverse roles and interactions of the SWI/SNF chromatin remodeling complex - Diverse roles and interactions of the SWI/SNF chromatin remodeling complex
Euskirchen GM, Auerbach RK, Davidov E, Gianoulis TA, Zhong G, Rozowsky J, Bhardwaj N, Gerstein MB, Snyder M. PLoS Genet. 2011 Mar;7(3):e1002008.