Anti-SMC1 antibody
| Name | Anti-SMC1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21583 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IP IHC-P |
| Species Reactivities | Mouse, Human, Rat, Chicken, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human SMC1 |
| Blocking Peptide | Human SMC1 peptide |
| Description | Rabbit Polyclonal |
| Gene | SMC1B |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-SMC1 antibody (ab21583) at 1 µg/mlLane 1 : 20ug HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µgLane 3 : 20ug HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SMC1 peptide (ab23863) at 1 µg/mlLane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 20 µg with Human SMC1 peptide (ab23863) at 1 µg/mlSecondaryGoat polyclonal to Rabbit IgG (Alexa Fluor® 680) (ab28446) at 1/10000 dilution Performed under reducing conditions.
SMC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to SMC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab21583.Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.Band: 150kDa: SMC1; Non specific - 41 and 42kDa: We are unsure as to the identity of this extra band.
ICC/IF image of ab21583 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab21583, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red). Panel A shows localisation of ab21583 to the nuclei, Panel B has the Alexa Fluor® 488 channel removed for comparison.
ab21583 staining SMC1 in assynchonous HeLa cells (green). Cells were paraformaldehyde-fixed (4% - 10min) and counterstained with DAPI (red). Secondary antibody: Goat anti-Rabbit conjugated to Cy3 ®. Please refer to Abreview for further details.See Abreview
IHC image of SMC1 staining in human skin FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21583, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Product References
Overall and allele-specific expression of the SMC1A gene in female Cornelia de - Overall and allele-specific expression of the SMC1A gene in female Cornelia de
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Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere - Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere
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Specific sites in the C terminus of CTCF interact with the SA2 subunit of the - Specific sites in the C terminus of CTCF interact with the SA2 subunit of the
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The human DEK oncogene regulates DNA damage response signaling and repair. - The human DEK oncogene regulates DNA damage response signaling and repair.
Kavanaugh GM, Wise-Draper TM, Morreale RJ, Morrison MA, Gole B, Schwemberger S, Tichy ED, Lu L, Babcock GF, Wells JM, Drissi R, Bissler JJ, Stambrook PJ, Andreassen PR, Wiesmuller L, Wells SI. Nucleic Acids Res. 2011 Sep 1;39(17):7465-76.