All lanes : Anti-SMC1 antibody [C2M] (ab16147) at 1/1000 dilutionLane 1 : Human BT-20 (breast cancer cell line) - whole cell lysateLane 2 : Human BT-474 (breast cancer cell line) - whole cell lysateLane 3 : Human HBL-100 (breast cancer cell line) - whole cell lysateLane 4 : Human HCC-1937 (breast cancer cell line) - whole cell lysateLane 5 : Human HME-1 (breast cancer cell line) - whole cell lysateLane 6 : Human Hs-578T (breast cancer cell line) - whole cell lysateLane 7 : Human MCF-10A (breast cancer cell line) - whole cell lysateLane 8 : Human MDA-MB 157(breast cancer cell line) - whole cell lysateLane 9 : Human MDA-MB 231 (breast cancer cell line) - whole cell lysateLane 10 : Human MDA-MB 436(breast cancer cell line) - whole cell lysateLane 11 : Human MDA-MB 453 (breast cancer cell line) - whole cell lysateLane 12 : Human MDA-MB 549 (breast cancer cell line) - whole cell lysateLane 13 : Human T47D (breast cancer cell line) - whole cell lysateLysates/proteins at 50 µg per lane.Second