Anti-SMC2 antibody
| Name | Anti-SMC2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10412 |
| Prices | $390.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IP IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human, Chimpanzee, Monkey, Ape, Orangutan |
| Antigen | Immunogen was a synthetic peptide, which represented a portion of human structural maintenance of chromosomes 2 encoded within exon 27 (LocusLink ID 10592) |
| Description | Rabbit Polyclonal |
| Gene | SMC2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab10412 (1/1000) staining SMC2 in assynchronous HeLa cells in conjunction with a secondary goat anti-rabbit antibody (1/200) conjugated to Cy3 ® (green). Cells were counterstained with DAPI to show the nucleus (red).See Abreview
IHC image of ab10412 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab10412, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colorectal tissue, staining SMC2 with ab10412 at 1/200 dilution. Staining was detected using DAB. N, normal tissue; T: tumor tissue (adenocarcinoma)
Product References
Ribosomal RNA gene transcription mediated by the master genome regulator protein - Ribosomal RNA gene transcription mediated by the master genome regulator protein
Huang K, Jia J, Wu C, Yao M, Li M, Jin J, Jiang C, Cai Y, Pei D, Pan G, Yao H. J Biol Chem. 2013 Sep 6;288(36):26067-77.
Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor - Senataxin, defective in the neurodegenerative disorder ataxia with oculomotor
Yuce O, West SC. Mol Cell Biol. 2013 Jan;33(2):406-17.
Human SMC2 protein, a core subunit of human condensin complex, is a novel - Human SMC2 protein, a core subunit of human condensin complex, is a novel
Davalos V, Suarez-Lopez L, Castano J, Messent A, Abasolo I, Fernandez Y, Guerra-Moreno A, Espin E, Armengol M, Musulen E, Ariza A, Sayos J, Arango D, Schwartz S Jr. J Biol Chem. 2012 Dec 21;287(52):43472-81.
53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of - 53BP1 nuclear bodies form around DNA lesions generated by mitotic transmission of
Lukas C, Savic V, Bekker-Jensen S, Doil C, Neumann B, Pedersen RS, Grofte M, Chan KL, Hickson ID, Bartek J, Lukas J. Nat Cell Biol. 2011 Mar;13(3):243-53.
Centromere DNA decatenation depends on cohesin removal and is required for - Centromere DNA decatenation depends on cohesin removal and is required for
Wang LH, Mayer B, Stemmann O, Nigg EA. J Cell Sci. 2010 Mar 1;123(Pt 5):806-13.
PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle - PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle
Liu W, Tanasa B, Tyurina OV, Zhou TY, Gassmann R, Liu WT, Ohgi KA, Benner C, Garcia-Bassets I, Aggarwal AK, Desai A, Dorrestein PC, Glass CK, Rosenfeld MG. Nature. 2010 Jul 22;466(7305):508-12.
Bcl-2 is an integral component of mitotic chromosomes. - Bcl-2 is an integral component of mitotic chromosomes.
Barboule N, Demeter K, Benmeradi N, Larminat F. Cell Biol Int. 2009 May;33(5):572-7.