Anti-Smg1 antibody
| Name | Anti-Smg1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab30916 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Mouse, Human, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 3600 - C-terminus of Human Smg1 |
| Description | Rabbit Polyclonal |
| Gene | SMG1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-Smg1 antibody (ab30916) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 10 µgLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) at 20 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.
ab30916 (1/200) staining Smg1 by ICC/IF in assynchronous HeLa Cells (green). Cells were countersatined with DAPI (red) in order to show the nucleus. Secondary antibody: Goat anti-Rabbit conjugated to Cy3®. Please refer to abreview for further details.See Abreview
ICC/IF image of ab30916 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab30916, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HeLa, Hek293 and MCF7 cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml
Product References
An atlas of chromatoid body components. - An atlas of chromatoid body components.
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Upf1 senses 3'UTR length to potentiate mRNA decay. - Upf1 senses 3'UTR length to potentiate mRNA decay.
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