SMG1

Gene Symbol SMG1
Entrez Gene 23049
Alt Symbol 61E3.4, ATX, LIP
Species Human
Gene Type protein-coding
Description SMG1 phosphatidylinositol 3-kinase-related kinase
Other Description PI-3-kinase-related kinase SMG-1|lambda-interacting protein|lambda/iota protein kinase C-interacting protein|serine/threonine-protein kinase SMG1|smg-1 homolog, phosphatidylinositol 3-kinase-related kinase
Swissprots Q8NFX2 Q96Q15 Q96RW3 O43305 Q96QV0 Q13284
Accessions EAW50260 EAW50262 Q96Q15 AB007881 BAA24851 AB061371 BAB70696 AF186377 AAK58892 AF395444 AAM73708 AK025794 AK057563 AK074359 AK123332 AL833298 AW088221 AW503964 AY014957 AAK00511 BC037335 BC047629 BC156497 AAI56498 BE079340 BF333707 BU633006 CA868047 DA433527 DA854035 U32581 AAA86535 XM_005255182 XP_005255239 XM_005255183 XP_005255240 XM_005255184 XP_005255241 XM_011545769 XP_011544071 XM_011545770 XP_011544072 NM_015092 NP_055907
Function Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR)
Subcellular Location Nucleus {ECO:0000269|PubMed:15175154}. Cytoplasm {ECO:0000269|PubMed:15175154}.
Tissue Specificity Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver. {ECO:0000269|PubMed:15175154, ECO:0000269|PubMed:8524286}.
Top Pathways mRNA surveillance pathway

SMG-1 (D42D5) Rabbit mAb - 9149 from Cell Signaling Technology

Prices $99.00, $246.00
Sizes 20 µl (2 western blots), 100 µl (10 western blots)
Host Rabbit
Clonality Monoclonal
Clone D42D5
Western blot analysis of extracts from U-2 OS, 293, and MCF7 cells using SMG-1 (D42D5) Rabbit mAb.

SMG-1 (V72) Antibody - 9592 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from HT-29, COS and U-2 OS cells using SMG-1 (V72) Antibody.

SMG-1 (Q25) Antibody - 4993 from Cell Signaling Technology

Prices $246.00
Sizes 100 µl (10 western blots)
Host Rabbit
Clonality Polyclonal
Western blot analysis of extracts from various cell types using SMG-1 (Q25) Antibody.

SMG1 (E-4) - sc-374557 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
SMG1 (E-4): sc-374557. Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic and nuclear localization.

SMG1 (H-300) - sc-98676 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal

SMG1 (T-18) - sc-79075 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

SMG1 (6-RE13) - sc-135563 from Santa Cruz Biotechnology

Prices $295.00
Sizes 100 µg/ml
Host Mouse
Clonality Monoclonal
SMG1 (6-RE13): sc-135563. Immunoperoxidase staining of formalin-fixed, paraffin-embedded human testis tissue showing nuclear and cytoplasmic localization.

Anti-Smg1 antibody - ab30916 from Abcam

Prices $380.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smg1 antibody (ab30916) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µgLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899) at 10 µgLane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate (ab7909) at 20 µgSecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilutionPerformed under reducing conditions.

Anti-Smg1 antibody - ab77304 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Anti-Smg1 antibody (ab77304) at 5 µg/ml + Recombinant tagged human Smg1 fragment at 0.2 µgSecondaryGoat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Anti-Smg1 antibody - ab80600 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Smg1 antibody (ab80600) at 1 µg/mlLane 1 : Whole HeLa cell lysate at 50 µgLane 2 : Whole HeLa cell lysate at 15 µgdeveloped using the ECL technique

Anti-Smg1 antibody - ab56967 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Western blot against tagged recombinant protein immunogen using ab56967 Smg1 antibody at 1ug/ml.

Anti-Smg1 antibody - ab56970 from Abcam

Prices $370.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-Smg1 antibody - ab70439 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Lanes 1 - 2 : Anti-Smg1 antibody (ab70439) at 1 µg/mlLanes 3 - 4 : Anti-Smg1 antibody (ab70439) at 0.4 µg/mlLanes 5 - 6 : Anti-Smg1 antibody (ab70439) at 0.1 µg/mlLane 1 : Nuclear extract from HeLa cells. at 5 µgLane 2 : Nuclear extract from HeLa cells. at 15 µgLane 3 : Nuclear extract from HeLa cells. at 15 µgLane 4 : Nuclear extract from HeLa cells. at 50 µgLane 5 : Nuclear extract from HeLa cells. at 15 µgLane 6 : Nuclear extract from HeLa cells. at 50 µgdeveloped using the ECL technique

Anti-Smg1 antibody - ab82548 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
ab82548, at a 1:250 dilution, staining human Smg1 in breast adenocarcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

Anti-Smg1 antibody [EPR4591(2)] - ab151730 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR4591(2)
All lanes : Anti-Smg1 antibody [EPR4591(2)] (ab151730) at 1/1000 dilutionLane 1 : Human fetal brain lysateLane 2 : Saos2 cell lysate Lane 3 : HT1080 cell lysateLane 4 : Human skeletal muscle lysateLane 5 : HeLa cell lysateLane 6 : 293T cell lysate Lysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution

Anti-SMG1 / ATX Antibody (C-Terminus) - LS-C46851 from LifeSpan Bioscience

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (aa2300-2350) - LS-C89136 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (aa1000-1050) - LS-C89135 from LifeSpan Bioscience

Prices $545.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (N-Terminus, AP) - LS-C282779 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (N-Terminus, Biotin) - LS-C282780 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (N-Terminus, Azide-free, HRP) - LS-C282784 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (N-Terminus, APC) - LS-C282783 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (N-Terminus, PE) - LS-C282785 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (N-Terminus, FITC) - LS-C282782 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SMG1 / ATX Antibody (C-Terminus, AP) - LS-C282786 from LifeSpan Bioscience

Prices $515.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
Search more