COBRA1 (D6K9A) Rabbit mAb
| Name | COBRA1 (D6K9A) Rabbit mAb |
|---|---|
| Supplier | Cell Signaling Technology |
| Catalog | 14894 |
| Prices | $246.00 |
| Sizes | 100 µl (10 western blots) |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | D6K9A |
| Applications | WB IP ChIP |
| Species Reactivities | Human, Mouse, Rat, Monkey, Hamster, Bovine |
| Antigen | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COBRA1 protein. |
| Description | Rabbit Monoclonal |
| Gene | NELFB |
| Supplier Page | Shop |
Product images
Western blot analysis of extracts from various cell lines using COBRA1 (D6K9A) Rabbit mAb.
Immunoprecipitation of COBRA1 from MCF7 cell extracts using Rabbit (DA1E) mAb IgG XP ® Isotype Control #3900 (lane 2) or COBRA1 (D6K9A) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using COBRA1 (D6K9A) Rabbit mAb.
Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 10 6 MCF7 cells grown in phenol red free medium and 5% charcoal stripped FBS for 4 d then treated with β-estradiol (10 nM) for 45 min and either 10 μl of COBRA1 (D6K9A) Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP ® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP ® Human c-Myc Intron 1 Primers #14905, human PMAIP1 promoter primers, and SimpleChIP ® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.