Anti-SMURF1 antibody
| Name | Anti-SMURF1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab57573 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment, corresponding to amino acids 165-269 of Human SMURF1 |
| Description | Mouse Monoclonal |
| Gene | SMURF1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
SMURF1 antibody (ab57573) used in immunohistochemistry at 2ug/ml on formalin fixed and paraffin embedded human stomach.
ICC/IF image of ab57573 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57573, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing MCF7 cells stained with ab57573 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57573, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_5/2450_SMURF1-Primary-antibodies-ab57573-2.jpg)
Overlay histogram showing MCF7 cells stained with ab57573 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57573, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Product References
Deubiquitinase FAM/USP9X interacts with the E3 ubiquitin ligase SMURF1 protein - Deubiquitinase FAM/USP9X interacts with the E3 ubiquitin ligase SMURF1 protein
Xie Y, Avello M, Schirle M, McWhinnie E, Feng Y, Bric-Furlong E, Wilson C, Nathans R, Zhang J, Kirschner MW, Huang SM, Cong F. J Biol Chem. 2013 Feb 1;288(5):2976-85.
Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad - Impaired phosphorylation and ubiquitination by p70 S6 kinase (p70S6K) and Smad
Wang J, Zhang Y, Weng W, Qiao Y, Ma L, Xiao W, Yu Y, Pan Q, Sun F. J Biol Chem. 2013 Nov 22;288(47):33667-81.