All lanes : Anti-SMURF1 antibody (ab94480) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : U2OS (Human osteosarcoma cell line) Whole Cell LysateLane 3 : MG-63 (Human osteosarcoma cell line) Whole Cell LysateLane 4 : Bone (Human) Tissue Lysate - tumor tissue (ab29359)Lane 5 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.