All lanes : Anti-SMYD1 antibody (ab32482) at 1 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257)Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330) with Human SMYD1 peptide (ab34470) at 1 µg/mlLane 4 : Testis (Human) Tissue Lysate - adult normal tissue (ab30257) with Human SMYD1 peptide (ab34470) at 1 µg/mlLysates/proteins at 10 µg per lane.SecondaryIRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilutionPerformed under reducing conditions.