All lanes : Anti-SMYD1 antibody ( ab32482 ) at 1 µg/ml Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue ( ab29330 ) Lane 2 : Testis (Human) Tissue Lysate - adult normal tissue ( ab30257 ) Lane 3 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue ( ab29330 ) with Human SMYD1 peptide (ab34470) at 1 µg/ml Lane 4 : Testis (Human) Tissue Lysate - adult normal tissue ( ab30257 ) with Human SMYD1 peptide (ab34470) at 1 µg/ml Lysates/proteins at 10 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution Performed under reducing conditions. Predicted band size : 56 kDa Observed band size : 56 kDa