Anti-SMYD3 antibody (ab155018) at 1/1000 dilution + 293T whole cell lysate at 30 µg
Confocal immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling SMYD3 with ab155018 at 1/500 dilution (green). Alpha-tubulin filaments were labeled with an Alpha-tubulin antibody at 1/2000 dilution (red).
Immunohistochemical analysis of paraffin-embedded Human hepatoma tissue labeling SMYD3 with ab155018 at 1/500 dilution.
ab155018 immunoprecipitates SMYD3 protein in IP experiments. IP Sample: 1000 µg HeLa whole cell lysate Lane 1. Control with 2.5 µg of preimmune rabbit IgG Lane 2. Immunoprecipitation of SMYD3 protein using 2.5 µg of ab155018. The immunoprecipitated SMYD3 protein was detected using ab155018 at 1/1000 dilution. 12% SDS-PAGE.