Anti-SMYD3 antibody [EPR11107(2)]

• Supplier: Abcam
• Catalog: ab187149
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR11107(2)
• Applications: WB IHC-P ICC/IF ICC/IF FC IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SMYD3 aa 250-350 (internal sequence)
• Description: Rabbit Monoclonal
• Gene: SMYD3
• Conjugate: Unconjugated

Product images

All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/20000 dilutionLane 1 : MCF-7 cell lysateLane 2 : HeLa cell lysateLane 3 : 293 cell lysateLane 4 : T47-D cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-SMYD3 antibody [EPR11107(2)] (ab187149) at 1/5000 dilutionLane 1 : Mouse brain tissue lysateLane 2 : Rat spleen tissue lysateLane 3 : NIH/3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human colonic carcinoma tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle tissue, labeling SMYD3 with ab187149 at 1/100 dilution. Counterstained with Hematoxylin.

Immunofluorescent analysis of 4% paraformaldehyde-fixed MCF-7 cells, labeling SMYD3 with ab187149 at 1/250 dilution (left image; green); secondary antibody was Goat anti rabbit IgG Alexa Fluor®488, and counterstained with Dapi (right image; blue).

Immunohistochemical analysis of 2% paraformaldehyde-fixed HeLa cells, labeling SMYD3 with ab187149 at 1/30 dilution. Secondary antibody was Goat anti rabbit IgG (FITC) at 1/150 dilution. Isotype control was a Rabbit monoclonal IgG.

Immunoprecipitation analysis of HeLa cell lysate labeling SMYD3 using ab187149 at 1/50 dilution (Lane 1). A Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody. Lane 2: PBS instead of HeLa lysate.