Anti-SMYD3 antibody - ChIP Grade

• Supplier: Abcam
• Catalog: ab16027
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ChIP WB IHC-P ICC/IF ICC/IF IHC-F
• Species Reactivities: Mouse, Human, Rat
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human SMYD3
• Blocking Peptide: Human SMYD3 peptide
• Description: Rabbit Polyclonal
• Gene: SMYD3
• Conjugate: Unconjugated

Product images

All lanes : Anti-SMYD3 antibody - ChIP Grade (ab16027) at 1 µgLane 1 : SW480 (Human colon adenocarcinoma cell line) Whole Cell LysateLane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 3 : SW480 (Human colon adenocarcinoma cell line) Whole Cell Lysate with Human SMYD3 peptide (ab16134) at 1 µg/mlLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate with Human SMYD3 peptide (ab16134) at 1 µg/mlLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Image courtesy of Human Protein Atlasab16027 staining SMYD3 in Human cervix. Paraffin embedded human uterine tissue was incubated with ab16027 (1/75) for 30 minutes at room tempetrature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab16027 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

Immunohistochemistical detection of SMYD3 protein using antibody ab16027 on PFA perfusion fixed mouse brain sections. Primary antibody used at 1/1000 for 18 hours @ 20°C in PBS + 0.3 % Triton X100.  Secondary antibody: goat anti-rabbit Alexa Fluor 488® (1/1000). This antibody produced a diffuse staining and some staining in cellular processesl; it is not clear if these cells are neurons or glial cells. The picture submitted shows the staining obtained at the level of the cortex and shows these cellular processes (dendrites??).See Abreview

ICC/IF image of ab16027 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab16027 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Product References

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