All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilutionLane 1 : Extracts from HT29 cells (5-30µg), minus immunising peptideLane 2 : Extracts from HT29 cells (5-30µg) plus immunising peptide (5-10µg)
All lanes : Anti-SNAIL + SLUG (phospho S246) antibody (ab63568) at 1/500 dilutionLane 1 : SNAIL (SNAI1) Human Recombinant Protein Lane 2 : SLUG (SNAI2) Human Recombinant ProteinLysates/proteins at 0.1 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
Immunofluorescent staining of HuvEc cells, using ab63568 at a 1/500 dilution, minus immunising peptide (left image) and plus immunising peptide (right image).